Status:

COMPLETED

Evaluate Tolerability of a Recombinant DNA HIV-1 Vaccine in Healthy Adults

Lead Sponsor:

St. Jude Children's Research Hospital

Collaborating Sponsors:

National Institutes of Health (NIH)

Conditions:

Human Immunodeficiency Virus

HIV Infections

Eligibility:

All Genders

18-40 years

Phase:

PHASE1

Brief Summary

This protocol will evaluate the safety and tolerability of the vaccine EnvDNA in healthy adults. DNA-based vaccines are being studied for the prevention of human immunodeficiency virus (HIV), malaria ...

Detailed Description

This is a research (investigational) study to find out about the safety of a new potential vaccine for HIV. This potential vaccine may eventually become a part of a sequence of three experimental vacc...

Eligibility Criteria

Inclusion

  • Healthy adults; age greater than 18 years, less than or equal to 40 years
  • HIV-1 negative as documented by negative ELISA and negative Western blot analysis within 30 days prior to immunization
  • Normal history and physical exam
  • Normal complete blood count and differential obtained within 60 days prior to immunization, and defined as:
  • hemoglobin greater than or equal to 12.0 gm/dl for females and greater than or equal to 14.0 gm/dl for males
  • white blood cell count greater than or equal to 3500 cells/mm3
  • platelet count between 150,000 and 550,000 cells/mm3
  • CD4+ T cell count greater than or equal to 400 cells/mm3 (if a single CD4+ T cell count \<400 cells/mm3 is obtained, a repeat count will be performed and immunization will proceed if the repeat count is greater than or equal to 400 cells/mm3)
  • Anti-nuclear antibody titer \<1:80 (by IFA) and negative anti-DNA antibody within 60 days prior to immunization
  • Negative for Hepatitis B surface antigen and Hepatitis C
  • AST and ALT within normal institutional limits obtained within 60 days prior to immunization
  • Serum creatinine, Na+, K+ and Cl- within normal institutional limits, obtained within 60 days prior to immunization
  • Serum creatine phosphokinase (CPK) within normal institutional limits obtained within 60 days prior to immunization
  • Not planning to become pregnant during study vaccinations and for three months after last vaccination
  • Availability for at least one year of follow-up

Exclusion

  • History of immunosuppressive illness, chronic illness (e.g. asthma, diabetes, hypertension, bleeding diathesis, etc), or
  • Receiving therapy or prophylaxis for tuberculosis
  • Medical or psychiatric condition or occupational responsibilities which preclude subject compliance with the protocol. Specifically excluded are persons with a history of suicide attempts, recent suicidal ideation or who have past or present psychosis
  • Live attenuated vaccines within 60 days of study enrollment (Note: subunit or killed vaccines \[e.g. influenza or pneumococcal\] are not exclusionary, but should be given at least 2 weeks before or after HIV immunization)
  • Use of experimental agents within 30 days prior to study enrollment
  • Receipt of blood products or immunoglobulin in the past 6 months
  • Subjects with known allergy to the antibiotic kanamycin
  • Pregnancy at the time of vaccination (urine pregnancy test)
  • Nursing mother at the time of vaccination
  • Any member of the Investigator's laboratory program
  • Participation in previous HIV vaccine trial

Key Trial Info

Start Date :

February 1 2005

Trial Type :

INTERVENTIONAL

Allocation :

ACTUAL

End Date :

November 1 2009

Estimated Enrollment :

6 Patients enrolled

Trial Details

Trial ID

NCT00187148

Start Date

February 1 2005

End Date

November 1 2009

Last Update

April 26 2017

Active Locations (1)

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St. Jude Children's Research Hospital

Memphis, Tennessee, United States, 38105