Status:

COMPLETED

Mechanisms Relating to the Distinct in Vitro Susceptibility of Human Macrophages to L. Viannia Infection

Lead Sponsor:

Yale University

Collaborating Sponsors:

National Institute of Allergy and Infectious Diseases (NIAID)

Conditions:

Leishmaniasis

Eligibility:

All Genders

18-70 years

Brief Summary

The purpose of this study is to determine how the body defends itself against Leishmania (Viannia), a parasite that can cause a skin infection and skin sores. Certain cells in the immune system act mo...

Detailed Description

This is a cross-sectional study that aims to determine the mechanisms that lead to the distinct in vitro susceptibility of human macrophages to L. Viannia infection, and their relationship to clinical...

Eligibility Criteria

Inclusion

  • For specific objectives 1, 3 and 4:
  • Historical cases of chronic disease:
  • Age between 18-70 years
  • Historic parasitologically confirmed, skin test positive cutaneous leishmaniasis (CL) patients who presented chronic CL lesions of \> 6 months duration at the time of diagnosis
  • No active lesions
  • Voluntary participation in the study
  • Written and signed Informed consent for HIV testing and participation in the study
  • Historical cases of recurrent disease:
  • Age between 18-70 years
  • Historic CL patients who have developed new parasitologically confirmed lesions after resolution of prior disease
  • No active lesions
  • Voluntary participation in the study
  • Written and signed Informed consent for HIV testing and participation in the study
  • Asymptomatic infection:
  • Age between 18-70 years
  • Resident of endemic area
  • No history or evidence of active or prior dermal leishmaniasis
  • Voluntary participation
  • Written and signed Informed consent for HIV testing and participation in the study
  • Healthy donors:
  • Age between 18-70 years
  • No history or evidence of exposure to transmission of leishmaniasis
  • Voluntary participation
  • Written and signed Informed consent for HIV testing and participation in the study
  • For specific objective 2:
  • Historical cases of chronic disease:
  • Age between 18-70 years
  • Historic parasitologically confirmed, skin test positive CL patients who presented chronic CL lesions of \> 6 months duration at the time of diagnosis
  • No active lesions
  • Voluntary participation in the study
  • Written and signed Informed consent for HIV testing and participation in the study
  • Historical cases of recurrent disease:
  • Age between 18-70 years
  • Historic CL patients who have developed new parasitologically confirmed lesions after resolution of prior disease
  • No active lesions
  • Voluntary participation in the study
  • Written and signed Informed consent for HIV testing and participation in the study
  • Asymptomatic infection:
  • Age between 18-70 years
  • Resident of endemic area
  • No history or evidence of active or prior dermal leishmaniasis
  • Voluntary participation
  • Written and signed Informed consent for HIV testing and participation in the study
  • Healthy donors:
  • Age between 18-70 years
  • No history or evidence of exposure to transmission of leishmaniasis
  • Voluntary participation
  • Written and signed Informed consent for HIV testing and participation in the study

Exclusion

  • Exclusion criteria for all groups (for specific objectives 1, 3 and 4):
  • Immunosuppressive disease
  • Pharmacotherapy with drugs that are immunosuppressive
  • Pregnancy
  • Breastfeeding
  • Unwillingness to participate
  • Exclusion criteria for all groups (for specific objective 2):
  • Weight below 50kg
  • Immunosuppressive disease (HIV)
  • Treatment with drugs that are immunosuppressive
  • Pregnancy
  • Breastfeeding

Key Trial Info

Start Date :

December 1 2006

Trial Type :

OBSERVATIONAL

Allocation :

ACTUAL

End Date :

April 1 2010

Estimated Enrollment :

34 Patients enrolled

Trial Details

Trial ID

NCT00401557

Start Date

December 1 2006

End Date

April 1 2010

Last Update

March 30 2020

Active Locations (1)

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Page 1 of 1 (1 locations)

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Centro Internacional de Entrenamiento e Investigaciones Medicas, CIDEIM

Cali, Colombia

Mechanisms Relating to the Distinct in Vitro Susceptibility of Human Macrophages to L. Viannia Infection | DecenTrialz