Status:

COMPLETED

Use of a Biofilm Antimicrobial Susceptibility Assay to Guide Antibiotic Therapy

Lead Sponsor:

The Hospital for Sick Children

Conditions:

Cystic Fibrosis

Eligibility:

All Genders

Phase:

PHASE2

Brief Summary

The purpose of this study is to determine whether choosing antibiotics based on a biofilm antimicrobial susceptibility assay rather than a conventional planktonic antimicrobial susceptibility assay to...

Detailed Description

Cystic fibrosis (CF) is the most common fatal genetic condition in the Caucasian population and affects over 3,000 Canadians. Respiratory failure caused by chronic pulmonary infection is the primary c...

Eligibility Criteria

Inclusion

  • Diagnosis of CF based on the following: sweat chloride \> 60 mEq/L or genotype with 2 identifiable mutations consistent with CF; and one or more clinical features consistent with CF
  • Chronically infected with P. aeruginosa (\>50% of respiratory specimens positive for P. aeruginosa in the 24 months prior to screening)
  • Able to produce sputum (expectorated or induced)
  • Able to reproducibility perform pulmonary function testing
  • Written informed consent provided

Exclusion

  • Sputum culture negative for P. aeruginosa or with a density of less that 10\^5 CFU/g at screening
  • Sputum culture positive for Burkholderia cepacia at screening
  • History of B. cepacia positive respiratory culture within 24 months prior to screening
  • Use of antibiotics other than those prescribed by the principal investigator
  • History of allergy (urticarial rash, diffuse erythroderma, serum sickness) to more than two groups of antibiotics (aminoglycosides, penicillins, cephalosporins, monobactams, macrolides, or quinolones) that are a therapeutic option
  • History of anaphylaxis or other life threatening complication to any antibiotic in the six groups that are a therapeutic option
  • Post lung transplantation or listed for lung transplantation
  • Pregnancy
  • A septic or clinically unstable patient
  • Presence of a condition or abnormality that in the opinion of an investigator would compromise the safety of the patient or the quality of the data

Key Trial Info

Start Date :

November 1 2008

Trial Type :

INTERVENTIONAL

Allocation :

ACTUAL

End Date :

March 1 2014

Estimated Enrollment :

134 Patients enrolled

Trial Details

Trial ID

NCT00786513

Start Date

November 1 2008

End Date

March 1 2014

Last Update

April 4 2014

Active Locations (5)

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Page 1 of 2 (5 locations)

1

BC Children's Hospital

Vancouver, British Columbia, Canada

2

St. Paul's Hospital

Vancouver, British Columbia, Canada

3

Hamilton Health Sciences

Hamilton, Ontario, Canada

4

St. Michael's Hospital

Toronto, Ontario, Canada