Status:

COMPLETED

A Study of Inhalation of 20,000 EU CCRE in Normal Volunteers Compared to Allergic Asthmatic Individuals

Lead Sponsor:

University of North Carolina, Chapel Hill

Collaborating Sponsors:

National Institute of Allergy and Infectious Diseases (NIAID)

Conditions:

Asthma

Hypersensitivity

Eligibility:

All Genders

18-50 years

Phase:

PHASE1

Brief Summary

This will be a single center, open label study comparing baseline characteristics of recovered sputum cells (collected on screening day) to those of cells recovered 6 hours after inhalational challeng...

Detailed Description

This will be a single center, open label study of allergic asthmatic and normal volunteers. The protocol will compare baseline characteristics of recovered sputum cells (collected on screening day) to...

Eligibility Criteria

Inclusion

  • Inclusion Criteria for healthy controls:
  • Normal lung function, defined as (Knudson 1976/1984 predicted set):
  • FVC of \> 80 % of that predicted for gender, ethnicity, age and height FEV1 of \> 80 % of that predicted for gender, ethnicity, age and height FEV1/FVC ratio of \> .75
  • Oxygen saturation of \> 94 % and normal blood pressure (Systolic between 150 - 90, Diastolic between 90-60 mm Hg)
  • Symptom Score no greater than 6 (out of a possible 24) for total symptom score with a value no greater than 2 for any one score.
  • Negative methacholine inhalation challenge as performed in the screening protocol. (Less than a 20% decrease in FEV1 at a maximum methacholine concentration of 10 mg/ml)
  • -Negative pregnancy test for females
  • Negative allergy skin test (AST)
  • Inclusion criteria for allergic asthmatics also include:
  • History of episodic wheezing, chest tightness, or shortness of breath after age of 6 years consistent with asthma, or physician diagnosed asthma after age of 6 years.
  • Positive methacholine test.
  • FEV1 of at least 80% of predicted and FEV1/FVC ratio of at least .70 (without use of bronchodilating medications for 12 hours)
  • Allergic sensitization to at least one of the following allergen preparations: (House Dust Mite f, House dust mite p, Cockroach, Tree mix, Grass Mix, Weed Mix, Mold Mix 1, Mold Mix 2, Rat, Mouse, Guinea Pig, Rabbit, Cat or Dog) confirmed by positive AST.
  • Negative allergy skin test as performed in the screening protocol.
  • Exclusion Criteria:
  • Any chronic medical condition considered by the PI as a contraindication to the exposure study including significant cardiovascular disease, diabetes requiring medication, chronic renal disease, or chronic thyroid disease.
  • Physician directed emergency treatment for an asthma exacerbation within the preceding 12 months.
  • Use of systemic steroid therapy within the preceding 12 months for an asthma exacerbation. All use of systemic steroids in the last year will be reviewed by a study physician.
  • Use of inhaled steroids, cromolyn or leukotriene inhibitors (montelukast or zafirlukast) except for use of cromolyn exclusively prior to exercise.
  • Use of daily theophylline within the past month.
  • Use of tricyclics and MAO inhibitors
  • Pregnancy or nursing a baby.
  • Cigarette smoking \> 1 pack per month.
  • Nighttime symptoms of cough or wheeze greater than 1x/week at baseline (not during a clearly recognized viral induced asthma exacerbation) which would be characteristic of a person of moderate or severe persistent asthma as outlined in the current NHLBI guidelines for diagnosis and management of asthma.
  • Exacerbation of asthma more than 2x/week which would be characteristic of a person of moderate or severe persistent asthma as outlined in the current NHLBI guidelines for diagnosis and management of asthma.
  • Daily requirement for albuterol due to asthma symptoms (cough, wheeze, chest tightness) which would be characteristic of a person of moderate or severe persistent asthma as outlined in the current NHLBI guidelines for diagnosis and management of asthma. (Not to include prophylactic use of albuterol prior to exercise).
  • Viral upper respiratory tract infection within 2 weeks of challenge.
  • Any acute infection requiring antibiotics within 2 weeks of challenge
  • Receipt of LAIV (Live Attenuated Influenza Vaccine), also know as FluMist®, within the prior 14 days

Exclusion

    Key Trial Info

    Start Date :

    January 1 2009

    Trial Type :

    INTERVENTIONAL

    Allocation :

    ACTUAL

    End Date :

    September 1 2012

    Estimated Enrollment :

    32 Patients enrolled

    Trial Details

    Trial ID

    NCT00839124

    Start Date

    January 1 2009

    End Date

    September 1 2012

    Last Update

    December 5 2012

    Active Locations (1)

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    UNC Center for Environmental Medicine, Asthma and Lung Biology

    Chapel Hill, North Carolina, United States, 27599-7310