Status:

COMPLETED

Augmentation of Dendritic Cell-Based Vaccines in Melanoma Patients by Depletion of Regulatory T Cells in Stage IV Melanoma Patients

Lead Sponsor:

Radboud University Medical Center

Collaborating Sponsors:

Dutch Cancer Society

Conditions:

Melanoma

Eligibility:

All Genders

18-75 years

Phase:

PHASE1

PHASE2

Brief Summary

Dendritic cells (DC) are the professional antigen presenting cells of the immune system. Multiple distinct DC lineage's exist and it is now well appreciated that the DC subset and the maturation stage...

Eligibility Criteria

Inclusion

  • Histologically documented evidence of melanoma.
  • Stage IV melanoma according to the 2001 AJCC criteria. \[53\] Limited tumor burden; LDH \< 2x upper limit of normal.
  • HLA-A2.1 phenotype according to lymphocyte HLA typing.
  • Expression of gp100 and/or tyrosinase on melanoma cells, as detected by immunohistochemistry, preferably on metastatic tumor, but if not available on primary tumor.
  • ECOG performance status 0-1, life expectancy \> 3 months.
  • Age 18-75 years. -
  • Written informed consent.
  • Expected adequacy of follow-up.
  • WBC \> 3.0 x 109/l, lymphocytes \> 0.8 x 109/l, platelets \> 100 x 109/l, serum creatinine \< 150 μmol/l, serum bilirubin \< 25 μmol/l.

Exclusion

  • No clinical signs of CNS metastases, in patients with a clinical suspicion of CNS metastases CT scan of the brain should be performed to exclude this.
  • No prior chemotherapy, immunotherapy, or radiotherapy within three months before planned vaccination is allowed.
  • No previous treatment with monoclonal antibodies.
  • No concomitant use of corticosteroids or other immunosuppressive agents.
  • No history of second malignancy within the last 5 years. Adequately treated basal carcinoma of skin or carcinoma in situ of cervix is acceptable within this period.
  • No serious concomitant disease, no active infections. Specifically, patients with autoimmune disease or organ allografts, and HBsAg or HIV positive patients are excluded.
  • Patients with a known allergy to shell fish (contains KLH) are excluded.
  • Patients with asthma or severe allergic disease necessitating medication are excluded
  • No pregnant or lactating women.

Key Trial Info

Start Date :

March 1 2004

Trial Type :

INTERVENTIONAL

End Date :

October 1 2005

Estimated Enrollment :

15 Patients enrolled

Trial Details

Trial ID

NCT00847106

Start Date

March 1 2004

End Date

October 1 2005

Last Update

February 19 2009

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