Status:

COMPLETED

An Investigation of Tumor Necrosis Factor (TNF)-Alpha in Asthma Using Biopsy Explants and Primary Bronchial Epithelial Cell Cultures

Lead Sponsor:

University Hospital Southampton NHS Foundation Trust

Collaborating Sponsors:

University of Southampton

Conditions:

Asthma

Eligibility:

All Genders

18-65 years

Brief Summary

We have developed in vitro systems including primary epithelial cell cultures and explant cultures of bronchial tissues to study the interaction between the bronchial mucosa and allergens. This approa...

Detailed Description

Volunteers with moderately severe asthma will be recruited and carefully characterized in terms of lung function, asthma symptom scores, medication usage and allergen sensitivity (by blood and skin te...

Eligibility Criteria

Inclusion

  • Inclusion Criteria:
  • Moderately severe asthmatics
  • Male or female
  • Uses inhaled beclomethasone 400-1000 mcg or equivalent
  • Documented positive skin test to common allergens and
  • FEV1 of \>60% predicted
  • Exclusion criteria:
  • Smokers
  • Subjects with an acute asthmatic episode in the last 6 weeks
  • Other significant clinical illnesses
  • Pregnant women
  • Those sensitive to lignocaine

Exclusion

    Key Trial Info

    Start Date :

    July 1 2000

    Trial Type :

    OBSERVATIONAL

    Allocation :

    ACTUAL

    End Date :

    June 1 2003

    Estimated Enrollment :

    12 Patients enrolled

    Trial Details

    Trial ID

    NCT01161303

    Start Date

    July 1 2000

    End Date

    June 1 2003

    Last Update

    July 13 2010

    Active Locations (1)

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    Southampton University Hospital Trust

    Southampton, Hampshire, United Kingdom, SO16 6YD