Status:

TERMINATED

Clinical Trial I/II Opened, Randomized and Controlled for the Study of the Use of Stem Cells Therapy in Insulinized Diabetic Patients Type 2 With Critical Ischemia in Lower Limbs (CLI): Study of the Needs of Insulin

Lead Sponsor:

Andalusian Initiative for Advanced Therapies - Fundación Pública Andaluza Progreso y Salud

Collaborating Sponsors:

Iniciativa Andaluza en Terapias Avanzadas

Conditions:

Critical Limb Ischemia (CLI)

Eligibility:

All Genders

18-85 years

Phase:

PHASE1

PHASE2

Brief Summary

To study the effect of the stem cells therapy autologous mononuclear from the bone marrow, autologous progenitor endothelial CD133 cells from the bone marrow and autologous mesenchymal stem cells from...

Detailed Description

To study the effect of the stem cells therapy autologous mononuclear from the bone marrow, autologous progenitor endothelial CD133 cells from the bone marrow and autologous mesenchymal stem cells from...

Eligibility Criteria

Inclusion

  • Age ≥18 and ≤ 85 years
  • Diabetic type 2, in treatment with insulin at least 3 previous months.
  • Vascular disease infrapopliteal atherosclerotic of severe degree (Patients with category Rutherford-Becker ≥ 4), pretty or bilateral. The critical ischemia of the foot is defined as a persistent / persistent pain that needs analgesia and / or not healing present sores\> 4 weeks, without evidence of improvement with conventional therapies and / or walking test between 1-6 minutes in two ergometries separated at least for 2 weeks and / or index ankle - arm in rest \<0,8.
  • Impossibility of revascularization surgical or endovascular or I fail in the surgery of revascularization performed at least 30 days before, with persistence or entry in phase of critical ischemia
  • Life expectancy \> 2 years.
  • Major amputation is not foreseen in any of the members in the next 6 months after the incorporation.
  • Normal Biochemical and bone marrow parameters defined for:
  • Leukocytes ≥ 3000
  • Neutrophils ≥ 1500
  • Platelets ≥ 140000
  • AST/ALT ≤ 2,5 standard range institution
  • Creatinine ≤2,5 mg/dl and explanation of creatinine ≤ 30 ml/min (MDRD4)
  • The women in fertile age will have to obtain negative results in a pregnancy test realized in the moment of the incorporation in the study and promise to use a contraceptive method medically approved while the study lasts.
  • Patients who give his Informed Consent in writing for the participation in the study. 10. Patients who have not taken part in another clinical trial in the last 3 months before the incorporation.

Exclusion

  • Precedent of neoplasia or hematologic disease (disease myeloproliferative, syndrome myelodysplasic or leukaemia) in the last 2 years.
  • Major previous amputation.
  • Patients with arterial uncontrolled hypertension (defined as arterial tension\> 180/110 in more than one occasion).
  • Cardiac severe insufficiency (NYHA the IVth) or Fraction of Ejection of left ventricle (FAITH) lower than 30 %.
  • Patients with ventricular malignant arrhythmias or unstable angina.
  • Diagnosis of venous deep thrombosis in 3 previous months.
  • Patients with genotype DD of the ECA or genotype E4 of the apo E.
  • Concomitant therapy that includes oxygen hyperbaric, vasoactive substances, agents against angiogenesis or inhibiting Cox-II. The agents use is allowed antiplatelets.
  • Index of corporal mass\> 40 Kg/m2.
  • Patients with the diagnosis of alcoholism in the moment of the incorporation.
  • Proliferative Retinopathy without possibility of treatment.
  • Diabetic Nephropathy with haemodialysis
  • Concomitant disease that reduces the life expectancy to less than 1 years.
  • Impossibility foreseen to obtain the inhaled one of 100ml of bone marrow.
  • Infection for HIV, Hepatitis B or Hepatitis C.
  • Impede in the follow-up.
  • ACV or heart attack of myocardium in the last 3 months
  • Anaemia (Haemoglobin \<7.9 g/dl)
  • Patients before treated with cellular therapy.

Key Trial Info

Start Date :

September 1 2011

Trial Type :

INTERVENTIONAL

Allocation :

ACTUAL

End Date :

July 1 2018

Estimated Enrollment :

20 Patients enrolled

Trial Details

Trial ID

NCT02287974

Start Date

September 1 2011

End Date

July 1 2018

Last Update

December 19 2018

Active Locations (2)

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Page 1 of 1 (2 locations)

1

Hopsital U. Virgen Macarena

Seville, Seville, Spain, 41007

2

CABIMER (Andalusian Center for Molecular Biology and Regenerative Medicine)

Seville, Spain, 41092

Clinical Trial I/II Opened, Randomized and Controlled for the Study of the Use of Stem Cells Therapy in Insulinized Diabetic Patients Type 2 With Critical Ischemia in Lower Limbs (CLI): Study of the Needs of Insulin | DecenTrialz