Status:
UNKNOWN
Relationship Between Nitric Oxide (NO) in Follicular Fluid and Sperm Fertilization Ability
Lead Sponsor:
IVI Murcia
Collaborating Sponsors:
Universidad de Murcia
Harvard School of Public Health (HSPH)
Conditions:
Male Infertility
Eligibility:
All Genders
18+ years
Phase:
NA
Brief Summary
Several studies indicate that Nitric Oxide (NO) plays an important role in the physiology of the reproductive system in mammals. It has been shown that NO affects sperm motility, it regulates the tyro...
Detailed Description
Main Objective: Determine changes in the NOS expression and protein nitrosylation in fertile and infertile patients, when adding L-Arginine, the substrate for NO synthesis, to the sperm capacitation ...
Eligibility Criteria
Inclusion
- This study will include fertile males such as the semen donors attending the clinic IVI Murcia and a group of subfertile males composed of patients, who attend the clinic to undergo an in vitro fertilization treatment and who possess one altered spermiogram parameter (according to the WHO 2010 guidelines). These participants will be recruited to examine the modifications in the NOS expression as well as the protein nitrosylation in fertile and subfertile men.
- This study will also include couples who undergo an IVF or ICSI treatment, in order to investigate if the effects of human follicular fluid on sperm capacitation are associated to NO levels in the fluid and to identify the correlation between NO levels in the follicular fluid and the clinical outcomes from ARTs.
Exclusion
- From this study will be excluded couples who undergo preimplantation genetic diagnosis, were diagnosed with repetitive abortion (three or more consecutive abortions before week 20) or implantation failure (lack of pregnancy after three embryo transfers with good quality embryos in women under 36 years of age or after two embryo transfers in women over 36 years of age), as well as women over 40 years of age.
Key Trial Info
Start Date :
September 21 2017
Trial Type :
INTERVENTIONAL
Allocation :
ESTIMATED
End Date :
October 1 2019
Estimated Enrollment :
82 Patients enrolled
Trial Details
Trial ID
NCT03307655
Start Date
September 21 2017
End Date
October 1 2019
Last Update
October 19 2017
Active Locations (1)
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1
IVI Murcia
Murcia, Spain, 30007