Status:
COMPLETED
Development of Oral Amino Acid Tracers to Study Protein Turnover in Humans
Lead Sponsor:
University of Toronto
Conditions:
Healthy Adult Males
Eligibility:
MALE
18-35 years
Phase:
NA
Brief Summary
Previous studies have used a combination of oral L-\[1-13C\]leucine and intravenous labeled L-\[5,5,5-2H3\]leucine to assess the acute postprandial changes in whole-body protein turnover (12, 19). Int...
Detailed Description
The primary objective of the proposed study is to validate the use of a novel oral tracer model to accurately and reliably measure myofibrillar protein synthesis. It is hypothesized that oral L-\[1-13...
Eligibility Criteria
Inclusion
- Male
- Currently performing structured physical activity 2-5 days per week
Exclusion
- Unable to safely perform exercise as per PARQ+ guidelines
- Currently using tobacco products
- Currently using or have history of anabolic steroid use
- Diagnosed with medical condition including type 2 diabetes, cancer, heart disease
- Unable to abstain from supplement use (HMB, branched chain amino acids, phosphatidic acid) for at least three weeks prior to trial
- currently using medications known to affect protein metabolism e.g. corticosteroids, NSAID, prescription-strength acne medication
- allergic to local anesthetics
- female: Hormonal fluctuations associated with the menstrual cycle have been reported to alter protein metabolism during exercise and may influence indices of the post-exercise myofibrillar protein synthetic response. Accordingly, the study will include males to ensure a stable hormonal environment and to increase the homogeneity of the physiological response.
Key Trial Info
Start Date :
February 25 2019
Trial Type :
INTERVENTIONAL
Allocation :
ACTUAL
End Date :
August 31 2019
Estimated Enrollment :
22 Patients enrolled
Trial Details
Trial ID
NCT04887727
Start Date
February 25 2019
End Date
August 31 2019
Last Update
May 14 2021
Active Locations (1)
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1
Goldring Centre for High Performance Sport at the University of Toronto
Toronto, Ontario, Canada, M5S2C9