Status:

NOT_YET_RECRUITING

An Exploratory Clinical Study Evaluating the Safety and Efficacy of Intravenous Anti-CD20/CD30-CAR-T Cell Infusion in Relapsed/Refractory Lymphoma Patients.

Lead Sponsor:

Shanghai Tongji Hospital, Tongji University School of Medicine

Conditions:

B Cell Malignancies

Eligibility:

All Genders

18-70 years

Phase:

EARLY_PHASE1

Brief Summary

This study is a single-center,open-label,single-dose clinical trial of anti-CD20/CD30-CAR-T cell therapy in relapsed/refractory B-cell tumor patients after lymphocyte depletion pre-treatment. In this...

Detailed Description

This study is a single-center, open-label, single-dose clinical trial of anti-CD20/CD30-CAR-T cell therapy in relapsed/refractory lymphoma patients after lymphocyte depletion pre-treatment. The study...

Eligibility Criteria

Inclusion

  • (1) Voluntary participation in the clinical study; complete understanding by self or legally authorized guardian, informed of the study, and signing the Informed Consent Form (ICF); willing and able to comply with all trial procedures.
  • (2) Age between 18 and 70 years.
  • (3) Patients refractory or relapsed after current standard treatments (including allogeneic or autologous hematopoietic stem cell transplantation), and unsuitable for other treatment options such as second hematopoietic stem cell transplantation. Refractory/relapsed lymphoma is defined as:
  • No response to first-line therapy (primary refractory disease, excluding subjects intolerant to first-line therapy):
  • \- Progression of Disease (PD) assessment after first-line treatment
  • Best response of Stable Disease (SD) after at least 4 cycles of first-line treatment (e.g., 4 cycles of RCHOP), with SD maintenance duration not exceeding 6 months after the last dose.
  • No response to second-line or subsequent therapies:
  • PD as best response to the most recent treatment regimen
  • Best response of SD after at least 2 cycles of last-line treatment, with SD maintenance duration not exceeding 6 months after the last dose.
  • Refractory post autologous stem cell transplantation (ASCT):
  • Disease progression or relapse ≤12 months after ASCT (relapsing subjects must have biopsy-proven relapse)
  • If salvage therapy is performed post-ASCT, subjects must have had no response or relapse after the last-line treatment.
  • Relapsed or refractory disease after two or more lines of systemic therapy.
  • (4) Lymphoma patients with target antigens meeting the following criteria:
  • CD20/CD30 double-positive lymphomas
  • Relapse after anti-CD19-CAR-T cell therapy, and CD20-positive lymphomas
  • Never received anti-CD19-CAR-T cell therapy, CD20-positive lymphomas
  • CD30-positive Hodgkin lymphoma.
  • (5) Included lymphoma subtypes:
  • DLBCL-NOS (Diffuse Large B-Cell Lymphoma, not otherwise specified)
  • Primary mediastinal large B-cell lymphoma (PMBCL)
  • Transformed follicular lymphoma (TFL), previously treated with follicular lymphoma chemotherapy, subsequently transformed into DLBCL, refractory disease
  • Mantle cell lymphoma
  • High-grade B-cell lymphoma
  • Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL)
  • Hodgkin lymphoma (HL).
  • (6) ECOG performance status ≤2.
  • (7) Expected survival of at least 12 weeks.
  • (8) Adequate venous access (for single collection), and no other contraindications for blood cell separation.
  • (9) Laboratory requirements at screening, with no hematologic evaluation within 7 days of receiving growth factors (long-acting granulocyte colony-stimulating factor (G-CSF/PEG-CSF) requires a 2-week interval):
  • Absolute neutrophil count ≥1.0×10\^9/L;
  • Hemoglobin ≥60 g/L (without red blood cell transfusion within 7 days);
  • Platelets ≥50×10\^9/L (CLL indication unrestricted);
  • Serum total bilirubin ≤1.5× upper limit of normal (ULN); or ≤3× ULN if liver tissue invasion by tumor;
  • Aspartate aminotransferase (AST), alanine aminotransferase (ALT) ≤2.5× ULN, AST/ALT ≤5× ULN if liver tissue invasion by tumor;
  • Creatinine \<1.5× ULN and estimated glomerular filtration rate ≥60 mL/minute.
  • (10) Left ventricular ejection fraction ≥45%, echocardiogram (ECHO) showing no clinically significant pericardial effusion (excluding minimal or physiological effusions), and no clinically significant findings on electrocardiogram.
  • (11) Baseline oxygen saturation \>92% without supplemental oxygen.
  • (12) Women of childbearing potential must have a negative serum or urine pregnancy test result (women who have undergone surgical sterilization or who are at least 2 years postmenopausal are not considered of childbearing potential).

Exclusion

  • Here is the translation of the exclusion criteria for participants in a clinical study:
  • Evidence of central nervous system lymphoma on brain MRI; active primary central nervous system DLBCL, unless CNS involvement has been effectively treated (i.e., participant is asymptomatic) and there has been a local treatment interval of \>4 weeks prior to enrollment.
  • Active central nervous system diseases such as epilepsy, cerebrovascular ischemia/hemorrhage, dementia, cerebellar diseases, or any autoimmune diseases with central nervous system involvement.
  • History of or concurrent malignancies other than CD19+ malignancies.
  • Clinically significant cardiac disease, or arrhythmias not controlled by medication.
  • Presence or suspicion of uncontrolled fungal, bacterial, viral, or other infections requiring intravenous antibiotics; simple urinary tract infections and uncomplicated bacterial pharyngitis are allowed.
  • Hepatitis B (positive hepatitis B surface antigen and hepatitis B DNA \>1000 copies/mL) and hepatitis C (positive hepatitis C antibody).
  • Presence of any indwelling catheters or drainage tubes (e.g., percutaneous nephrostomy tube, Foley catheter, bile drainage tube, pleural/peritoneal/pericardial catheter); specialized central venous access devices like Port-A-Cath® or Hickman® catheters are allowed.
  • Use of the following medications prior to:
  • 1\) Ibrutinib within 1 day before apheresis. 2) Idelalisib (oral PI3Kδ inhibitor) within 2 days before apheresis. 3) Short-acting targeted therapy (such as tyrosine kinase inhibitors) within 72 hours before apheresis.
  • 4\) Venetoclax (BCL-2 inhibitor) within 4 days before apheresis. 5) Long-acting growth factors (such as pegfilgrastim) within 14 days before apheresis, or short-acting growth factors or mobilizing agents (such as granulocyte colony-stimulating factor (G-CSF)/filgrastim) within 5 days before apheresis.
  • 6\) Pharmacologic doses of corticosteroid therapy (\>5 mg/day prednisone or equivalent) and other immunosuppressive drugs within 7 days before enrollment.
  • 7\) Radiotherapy within 14 days before enrollment. 8) Systemic cytotoxic drugs within 14 days before enrollment, including daily or weekly low-dose maintenance chemotherapy (e.g., cyclophosphamide, fludarabine, bendamustine, chlorambucil, methotrexate, vinblastine).
  • If bridging therapy is administered post-apheresis, there must be at least a 7-day interval between bridging therapy and CAR-T cell infusion.
  • 9\) Anti-PD1 or anti-PDL1 therapy within 4 weeks before enrollment. 10) Vaccination within 4 weeks before enrollment. 11) Donor lymphocyte infusion (DLI) within 4 weeks before enrollment. 12) Immunostimulatory or immunosuppressive therapy within 3 months before enrollment (such as interferon-α, interferon-β, IL-2, lenalidomide, efalizumab, alemtuzumab, cyclosporine, or methotrexate).
  • (9) Active graft-versus-host disease (GVHD) using the CIBMTR acute GVHD grading system ≥ grade 2 or requiring systemic steroids greater than physiological doses.
  • (10) History in the past 2 years of autoimmune diseases causing end-organ damage or requiring systemic immunosuppressive/disease-modifying agents, such as Crohn's disease, rheumatoid arthritis, systemic lupus erythematosus.
  • (11) History in the past 12 months of myocardial infarction, cardiac vascular procedures or stent implantation, unstable angina, or other clinically significant cardiac diseases.
  • (12) History of genetic syndromes with bone marrow failure, such as Fanconi anemia, Costello syndrome, Shwachman-Diamond syndrome.
  • (13) Symptomatic deep vein thrombosis or pulmonary embolism requiring systemic anticoagulation therapy within 6 months before enrollment. Subjects requiring prophylactic anticoagulation are allowed.
  • (14) History of concurrent or prior malignancies (excluding basal cell carcinoma of the skin, in situ carcinoma of the breast/cervix, and other malignancies effectively controlled without treatment within the past five years).
  • (15) Use of other investigational medicinal products within 30 days before screening.
  • (16) Pregnant or lactating women of childbearing potential. Women who have undergone surgical sterilization or who are at least 2 years postmenopausal are not considered of childbearing potential.
  • (17) Participants unwilling to use contraception from agreeing to treatment until completion of lymphocyte depletion chemotherapy or CAR-T cell infusion within 12 months (whichever is longer).
  • (18) Any medical activities that could potentially interfere with the safety or efficacy evaluation of the study treatment.
  • (19) According to the investigator's judgment, participants who are unlikely to complete all study visits or procedures required by the protocol (including follow-ups), or comply with the requirements of participating in the study.

Key Trial Info

Start Date :

July 31 2024

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

July 30 2027

Estimated Enrollment :

12 Patients enrolled

Trial Details

Trial ID

NCT06519344

Start Date

July 31 2024

End Date

July 30 2027

Last Update

July 25 2024

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