Status:

RECRUITING

Safety and Early Efficacy of iPSC-Derived Motor Neuron Progenitor Cells (XS228) in Subacute Spinal Cord Injury: A Phase I Trial

Lead Sponsor:

XellSmart Bio-Pharmaceutical (Suzhou) Co., Ltd.

Collaborating Sponsors:

Third Affiliated Hospital, Sun Yat-Sen University

Conditions:

Spinal Cord Injury

Safety

Eligibility:

All Genders

18-65 years

Phase:

PHASE1

Brief Summary

This Phase I clinical trial is designed to evaluate the safety, tolerability of XS228 ( iPSC-Derived Motor Neuron Progenitor Cells) in patients with Subacute Spinal Cord Injury

Eligibility Criteria

Inclusion

  • Age: 18 to 65 years (inclusive), regardless of gender.
  • Etiology: Cervical (C4) to lumbar (L2) spinal cord injury (SCI) caused by traumatic injury or surgery-related factors.
  • Severity:
  • Classified as ASIA Impairment Scale (AIS) Grades A, B, or C. MRI-confirmed evidence of spinal cord injury.
  • Disease Stage:
  • Primary SCI occurring 14 to 60 days prior to screening (subacute phase).
  • Contraception:
  • Participants of childbearing potential (male and female) must agree to use effective non-hormonal contraceptive methods during the trial and for 6 months after trial completion.
  • Compliance:
  • Voluntarily participate in the clinical study. Ability to understand and comply with study procedures. Participant or legal guardian can provide written informed consent.

Exclusion

  • Neurological Inability
  • Primary spinal cord injury (SCI) during screening with concomitant severe traumatic brain injury precluding neurological function assessment.
  • Respiratory/Circulatory Instability
  • High cervical SCI (C1-C3) causing respiratory/circulatory compromise requiring endotracheal intubation or tracheostomy.
  • Life-Threatening Multiorgan Dysfunction
  • Concurrent severe injuries to other organ systems with life-threatening dysfunction.
  • Unstable Thoracoabdominal Injuries
  • Injuries to lungs, liver, kidneys, spleen, etc., deemed unstable by the investigator.
  • Prior Spinal Pathology
  • History of SCI or coexisting spinal disorders (e.g., ankylosing spondylitis, spinal deformities, primary/metastatic spinal tumors, spinal vascular malformations, syringomyelia).
  • Local Infection/Increased ICP
  • Active infection at the lumbar puncture site or intracranial hypertension during screening.
  • Severe Infections
  • Sepsis, septic shock, or severe pneumonia (per IDSA/ATS 2007 diagnostic criteria).
  • Confounding Neurological/Psychiatric Conditions
  • Parkinson's disease, severe dementia, myasthenia gravis, stroke, Guillain-Barré syndrome, diabetic neuropathy, or other conditions interfering with study assessments.
  • Cardiac Abnormalities (any of the following):
  • Congestive heart failure (NYHA Class III/IV). Severe uncontrolled arrhythmias (e.g., sick sinus syndrome, third-degree AV block).
  • Unstable angina or acute myocardial infarction within 3 months prior. Pulmonary Complications
  • Pulmonary hypertension, pulmonary embolism, or suspected embolism during screening.
  • Uncontrolled Hypertension/Hypotension
  • Systolic BP \>160 mmHg or diastolic BP \>100 mmHg; or systolic BP \<90 mmHg or diastolic BP \<60 mmHg.
  • Active Autoimmune Diseases
  • Requiring immunosuppressants (e.g., uncontrolled hyperthyroidism, systemic lupus erythematosus).
  • Immunosuppressant Non-Compliance
  • Unwillingness or inability to use immunosuppressants per protocol.
  • Laboratory Abnormalities (any of the following):
  • ALT/AST \>2×ULN or total bilirubin \>2×ULN. eGFR \<60 mL/min/1.73m² (CKD-EPI 2021 formula). APTT/PT \>2.5×ULN (without anticoagulants). Platelets \<100×10⁹/L or hemoglobin \<90 g/L. Allergy
  • History of severe allergies or hypersensitivity to trial drug/excipients (human albumin, lactated Ringer's solution).
  • Infectious Diseases
  • HBsAg+ with HBV DNA \>1000 IU/mL; HCV-Ab+; HIV-Ab+; or TP-Ab+. Lumbar Puncture Refusal
  • Unwillingness to undergo intrathecal administration procedures. Pregnancy/Lactation
  • Females who are pregnant or breastfeeding. Malignancy
  • Active malignancy or anticancer therapy within 5 years prior. Recent Clinical Trial Participation
  • Enrollment in another drug trial within 3 months prior. Investigator Discretion
  • Any condition deemed unsuitable for participation by the investigator.

Key Trial Info

Start Date :

July 2 2025

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

May 30 2028

Estimated Enrollment :

12 Patients enrolled

Trial Details

Trial ID

NCT06976229

Start Date

July 2 2025

End Date

May 30 2028

Last Update

November 25 2025

Active Locations (1)

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1

The Third Affiliated Hospital of Sun Yat-sen University

Guangzhou, Guangdong, China, 510630