Inclusion Criteria:

• Subjects are nonpregnant females ≥ 18 years and ≤ 42 years of age.
• Subjects obtain greater than or equal to 6 mature oocytes at the time of oocyte retrieval for ICSI or greater than or equal to 8 oocytes at the time of oocyte retrieval for standard insemination.
• Subjects are utilizing standard insemination or ICSI for fertilization.
• Subjects are undergoing PGT-A (PGT for aneuploidy) or PGT-M (PGT for monogenic disorders).
• Subjects are willing to comply with study protocol and procedures and provide written informed consent.

Exclusion Criteria:

• Subjects are utilizing donor oocytes, donor sperm, or gestational carrier.
• Subjects have a diagnosis of cryptozoospermia (no spermatozoa identified in fresh semen sample).
• Subjects are utilizing surgically removed sperm (e.g. via testicular sperm aspiration [TESA] or microsurgical epididymal sperm aspiration [MESA]).
• Subjects are utilizing frozen/thawed sperm, including in cases in which a fresh sample was planned and the sample is insufficient for use.
• Subjects are utilizing frozen/thawed oocytes.
• Subjects undergoing PGT-SR (PGT for structural rearrangements).
• Subjects are undergoing a day 3 (cleavage stage) embryo transfer.
• Subjects obtain less than 6 mature oocytes at the time of oocyte retrieval for ICSI or less than 8 oocytes at the time of oocyte retrieval for standard insemination.
• Subjects obtain greater than or equal to 6 mature oocytes at the time of oocyte retrieval for ICSI or greater than or equal to 8 oocytes at the time of oocyte retrieval for standard insemination but choose to fertilize fewer than 6 (for ICSI) or 8 (for standard insemination).
• Male partner is unable to produce a second semen sample within three hours of the first semen sample (standard abstinence sample)
• Male partner has an infectious disease.