Status:
COMPLETED
Microbiological Description of Sperm and Follicular Fluid Samples. Impact on IVF/ICSI Success Rates
Lead Sponsor:
GCS Ramsay Santé pour l'Enseignement et la Recherche
Conditions:
In Vitro Fertilization (IVF)
Medically Assisted Procreation (MAP)
Eligibility:
All Genders
18-59 years
Brief Summary
The goal of this observational study is to quantify and identify bacteria that may be present in semen and follicular fluid samples on the day of the IVF-ICSI attempt. The secondary objective is to :...
Detailed Description
This is a single-center observational study based on data contained in the medical records of couples treated and evaluated as part of Medically Assisted Procreation (IVF-ICSI). This study was conduc...
Eligibility Criteria
Inclusion
- Man aged 18 to 59 who have provided a sperm sample after an abstinence period of between 2 and 7 days. Sperm prepared by density gradient on day 0. Negative sperm culture (\<6 months). No specific urogenital history (varicocele, cryptorchidism, testicular ectopia, etc.).
- Women aged 18 to 43 undergoing oocyte puncture with collection of follicular fluid on day 0. No endometriosis or hydrosalpinx. Negative vaginal sample (\< 6 months).
- Negative HIV, HBV, and HCV serology tests within the last year.
- Absence of urinary tract infections in men and sexually transmitted infections (HPV, HSV, HIV-1/2, C. trachomatis, Gonorrhea, Syphilis, etc.) (\<6 months).
- Absence of recent antibiotic or antifungal treatment (end of treatment \< 3 months) Absence of probiotic intake (\< 3 months).
- No history of chemotherapy or radiation therapy.
- Embryonic culture performed up to day 6 for all zygotes.
Exclusion
- Homosexual patient couples or transsexual couples
- Patients treated for medically assisted procreation with a third-party donor
- Frozen sperm pellets (ejaculate or surgical)
- Donor sperm
- Sperm prepared by washing
- Abstinence period \<2 or \>7 days
- Fever \<3 months
- History of chemotherapy or radiotherapy.
- History of urinary tract infections in man (\< 6 months)
- History of sexually transmitted infections (HPV, HSV, HIV-1/2, C. trachomatis, gonorrhea, syphilis, etc.) (\<6 months)
- Specific urogenital history (varicocele, cryptorchidism, testicular ectopia, etc.)
- Recent antibiotic therapy or antifungal treatment (end of treatment \< 3 months)
- Taking probiotics (end of treatment \< 3 months)
- Positive sperm culture \< 6 months.
- Positive vaginal sample \< 6 months. White puncture.
- Collection failure.
- Fertilization failure
Key Trial Info
Start Date :
January 9 2024
Trial Type :
OBSERVATIONAL
Allocation :
ACTUAL
End Date :
March 29 2025
Estimated Enrollment :
50 Patients enrolled
Trial Details
Trial ID
NCT07187414
Start Date
January 9 2024
End Date
March 29 2025
Last Update
September 23 2025
Active Locations (1)
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1
Hôpital Privé de Parly II
Le Chesnay, France, 78150