Status:

NOT_YET_RECRUITING

YOLT-204 in Patients With Hemoglobinopathies

Lead Sponsor:

Guangzhou Women and Children's Medical Center

Conditions:

Hemoglobinopathies (Transfusion-dependent β-thalassemia and Sickle Cell Disease)

Eligibility:

All Genders

3-17 years

Phase:

EARLY_PHASE1

Brief Summary

This is a single-arm, open-label, single-dose, dose-escalation trial that plans to enrol 3-18 patients with transfusion-dependent β-thalassaemia (TDT) or sickle-cell disease (SCD). Its primary aims ar...

Eligibility Criteria

Inclusion

  • Aged 3-17 years (inclusive); any sex.
  • The subject and/or his/her legally authorized guardian/representative must fully understand the study and voluntarily sign a written informed-consent form.
  • Karnofsky Performance Status (KPS) ≥ 70 (if ≥ 16 years old) or Lansky Performance Scale (LPS) ≥ 70 (if \< 16 years old).
  • Detailed medical records of red-cell transfusions during the 2 years before informed-consent signature must be available, including volume or units transfused and pre-/post-transfusion red-cell and hemoglobin levels.
  • No severe hematopoietic dysfunction; cardiac, pulmonary, hepatic, and renal function essentially normal.
  • Coagulation: international normalized ratio (INR) and activated partial thromboplastin time (APTT) ≤ 1.5 × upper limit of normal (ULN).
  • Renal function: serum creatinine ≤ 1.5 × ULN; if creatinine \> 1.5 × ULN, calculated creatinine clearance \> 50 mL/min by the Schwartz formula.
  • Hepatic function: alanine aminotransferase (ALT) ≤ 3 × ULN and aspartate aminotransferase (AST) ≤ 3 × ULN.
  • Cardiac function: left-ventricular ejection fraction (LVEF) ≥ 50 %.
  • Good compliance; willing to adhere to visit schedules, study procedures, laboratory tests, and other protocol requirements.
  • Agrees to use at least one highly effective contraceptive method from informed-consent signature through the end of the main study (Week 52 visit).
  • Willing to participate in long-term follow-up.
  • Screening genotype shows HbSS or HbSβ0; prior reports acceptable if assessed as adequate by the investigator.
  • If on L-glutamine, regimen must have been stable for ≥ 3 months before study-drug administration; if on hydroxyurea, must have discontinued ≥ 8 weeks before study-drug administration.
  • Meets severe SCD criteria: despite optimal supportive therapy (including, but not limited to, analgesics and hydroxyurea), at least two of the following events occurred in the 12 months before screening:
  • Severe intermittent acute pain requiring healthcare-provider management;
  • Acute chest syndrome with new pulmonary infiltrate on chest imaging plus pneumonia-like symptoms, pain, or fever;
  • Splenic sequestration crisis manifested by enlarged spleen, left upper-quadrant pain, and acute Hb drop \> 20 g/L.

Exclusion

  • History of multiple drug allergies or hypersensitivity to oligonucleotides or lipid nanoparticles (LNP).
  • Clinically significant active bacterial, viral, fungal, or parasitic infection at screening, as judged by the investigator.
  • White blood cell (WBC) count \< 3 × 10⁹/L and/or platelet count \< 100 × 10⁹/L at screening.
  • Uncorrected bleeding diathesis. 5.Massive splenomegaly at screening (spleen edge below the umbilicus or \> 4 cm below the costal margin) deemed by the investigator to preclude enrollment.
  • Serum ferritin ≥ 5 000 ng/mL, or MRI T2\* evidence of severe cardiac or hepatic iron overload.
  • Positive for hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibody, anti-HIV antibody, or specific anti-Treponema pallidum antibody.
  • Prior hematopoietic stem-cell transplantation, gene therapy, or gene-editing therapy.
  • Participation in another clinical trial and receipt of investigational product within 3 months before first dose of study drug.
  • Current or prior malignancy, myeloproliferative disorder, or immunodeficiency disease.
  • Severe psychiatric illness precluding cooperation; clinically significant pulmonary hypertension requiring medical intervention; recent malaria; first-degree relative with hematologic malignancy.
  • Positive pregnancy test, pregnancy, or lactation in female subjects at screening.
  • Any condition (past or present) that, in the investigator's opinion, could confound results, compromise participation, or render the patient unsuitable for the study.
  • Use within 3 months before study drug: erythropoietin (EPO), thalidomide, hydroxyurea, luspatercept, or similar agents.
  • In subjects ≥ 12 years, abnormal transcranial Doppler (TCD) with middle cerebral or internal carotid artery velocity ≥ 200 cm/s.
  • History of moyamoya disease or imaging findings consistent with moyamoya at screening, assessed by the investigator as conferring bleeding risk.

Key Trial Info

Start Date :

September 30 2025

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

April 30 2027

Estimated Enrollment :

18 Patients enrolled

Trial Details

Trial ID

NCT07190001

Start Date

September 30 2025

End Date

April 30 2027

Last Update

September 24 2025

Active Locations (1)

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1

Guangzhou women and children's medical center

Guangzhou, Guangdong, China, 510405