Status:

NOT_YET_RECRUITING

Investigation of the Efficacy of a Probiotic Mixture in Moderate Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD): A Mechanistic Trial

Lead Sponsor:

AB Biotics, SA

Conditions:

MASLD - Metabolic Dysfunction-Associated Steatotic Liver Disease

Fatty Liver Disease, Nonalcoholic

Eligibility:

All Genders

18-65 years

Phase:

NA

Brief Summary

The goal of this clinical trial is to evaluate whether a specific probiotic mixture can improve liver health in adults with moderate metabolic dysfunction-associated steatotic liver disease (MASLD). ...

Eligibility Criteria

Inclusion

  • Patients aged from 18 to 65 years old
  • BMI 25.34,9kg/m2
  • Diagnosed with MASLD and CAP value \> 268 dB/m evaluated by FibroScan®
  • High ALT levels (\>43 U/L in males and \>33 U/L in females)
  • Having at least three of the following features compatible with metabolic syndrome:
  • i. Waist circumference ≥ 102 cm in males and ≥ 88 cm in females. ii. Fasting serum glucose (≥ 5.6 mmol/L or 100 mg/dl). iii. Glycated haemoglobin (HbA1c ≥ 5.7%/ 39 mmol/L). iv. Diagnosed or treated for type 2 diabetes. v. High blood pressure (≥ 130/85 mmHg). vi. High plasma triglycerides (≥ 1.70 mmol/L or 150 mg/dl). vii. Lower plasma of High-Density Lipoprotein (HDL cholesterol) (≤ 1.0 mmol/L or 40 mg/dl for males and ≤ 1.3 mmol/L or 50 mg/dl for females).
  • Stable weight in the last 3 months (less than ± 4% weight variation).
  • Stable medication or intake of food supplements for a medical condition that can affect study outcomes according to the Investigator's judgement in the last three months prior to study entry (bile salt sequestrants are not permitted).
  • Not planning to change their dietary and lifestyle habits during the study.
  • Willing and able to provide informed consent and comply with study procedures.

Exclusion

  • Fibrosis scores equal or higher than F2 (≥ 7.5 kPa).
  • History of acute or chronic hepatitis A, B or C, autoimmune hepatitis, drug-induced liver diseases, severe liver diseases.
  • Prior or pending liver transplantation.
  • Patients with at least one of the following concurrent conditions:
  • i. Type I diabetes ii. Uncontrolled type II diabetes (HbA1c \>8%) iii. Hypertriglyceridemia \> 350mg/dl iv. Human Immunodeficiency Virus (HIV) infection v. Diagnosis of hemochromatosis
  • Current use of pioglitazone, SGLT-2 inhibitors, or approved drugs for MASLD or steatohepatitis within 8 weeks.
  • Current use of bile salt sequestrants within 8 weeks.
  • Significant gastrointestinal disease, such as inframmatory bowel disease (IBD, short bowel syndrome, chronic or recurrent diarrhoea, coeliac condition.
  • Pancreatic failure, biliary dysfunction (including cholecystectomy and blood bilirubin abnormalities)
  • Thyroid dysfunction, as assessed by the investigator (clinical criteria)
  • History of:
  • i. Cardiovascular disease (ischemic heart disease, heart failure, cerebrovascular disease, periphreal vascular disease).
  • ii. Cancer or immunosuppression. iii. Gastrointestinal surgery in the previous year (with the exception of appendicitis).
  • Patients with a history of chronic alcohol or drug abuse: \> 14 units/week for females and \> 21 units/week for male
  • Chronic and heavy smoking (\>20 cigarettes a day)
  • Regular intake (\> 3 days/week) of other probiotics (including food complements or diary foods with other probiotic strains, e.g. Activia®, Actimel® or similar).
  • Intake of nutraceuticals with an effect on hepatic function such as \> 500 mg/day omega-3 fatty acids, high-dose vitamin E supplements or milk thistle (sylibum marianum) extract ot their active ingredients (silymarin, silybin) regularly (\> 7 days) in the 15 days before study entry.
  • Current use of systemic corticosteroids, androgens, clopidogrel, digoxin, acenocoumarol, warfarin, phenytoin, topiramate, lithium, tricyclic antidepressants, MAOIs or second-generation antipsychotics, amiodarone, tamixofen and/or diltiazem.
  • History of use (\> 3 days) of oral or parenteral antibiotics one month before the study initiation.
  • Chronic use of laxatives.
  • Debilitating illnesses (advanced liver or kidney disease, severe depression, psychotic symptoms, neurological diseases).
  • Current pregnancy (positive urine test) or planning to become pregnant during the study.
  • Breastfeeding at the time of eligibility assessment.
  • Patients who have participated in a clinical trial in the six-month period before the study.

Key Trial Info

Start Date :

October 13 2025

Trial Type :

INTERVENTIONAL

Allocation :

ESTIMATED

End Date :

June 1 2027

Estimated Enrollment :

60 Patients enrolled

Trial Details

Trial ID

NCT07193927

Start Date

October 13 2025

End Date

June 1 2027

Last Update

October 3 2025

Active Locations (1)

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Hospital Universitari Dr. Josep Trueta

Girona, Girona, Spain, 17190