Status:

RECRUITING

Maternal Probiotic Intervention to Improve Gut Health-Trial II-Pakistan

Lead Sponsor:

Aga Khan University

Collaborating Sponsors:

Bill and Melinda Gates Foundation

International Centre for Diarrhoeal Disease Research, Bangladesh

Conditions:

Environmental Enteric Dysfunction (EED)

Stunting

Eligibility:

FEMALE

18-49 years

Phase:

PHASE2

Brief Summary

Burden: Environmental Enteric Dysfunction (EED) is an enteropathic condition characterised by altered gut permeability, infiltration of immune cells, and changes in villous architecture and cell diffe...

Detailed Description

A total of 144 pregnant women in their second trimester will be enrolled. Subsequently, 96 will be randomized to receive an antibiotic for 5 days, followed by a 1-day washout period (1 week), and then...

Eligibility Criteria

Inclusion

  • Inclusion criteria
  • Women aged 18 years or older in their first or early second trimester of pregnancy (13-17 weeks of gestational age \[GA\]), living in defined geographical areas of Bangladesh (Matlab), Pakistan, Zambia, and Burkina Faso, where it can be assumed that environmental enteropathy is prevalent
  • Presence of any 2 out of 11 selected bacterial pathogen targets (Aeromonas, Campylobacter coli, Campylobacter jejuni, Campylobacter Pan, Enteroaggregative Escherichia coli, Enteropathogenic Escherichia coli, Enterotoxigenic Escherichia coli, Plesiomonas, Shigella\_EIEC, Salmonella and Klebsiella pneumoniae in fecal samples measured by TAC-qPCR.
  • Presence of any of the following WASH conditions -
  • use surface water, unimproved water, or limited water for drinking; OR
  • use surface water, unimproved water, or limited water for cooking; OR
  • use surface water, unimproved water, or limited water for washing utensils; OR
  • practice open defecation, use unimproved sanitation (toilet facility), or limited sanitation (toilet facility); OR
  • lack facility or have limited facility for handwashing
  • Exclusion criteria
  • Potential participants will not be enrolled if they:
  • have MUAC ≥30 cm
  • are carrying more than one fetus (i.e., multiple pregnancy)
  • have diarrhea, defined as the passage of three or more loose stools per 24 hours, or have had diarrhea in the preceding 14 days
  • have fever or an active infection
  • have taken antibiotics or probiotics in the preceding 14 days
  • have taken steroids or non-steroidal anti-inflammatory drugs in the preceding 14 days
  • have severe anemia as determined using finger stick Hb \< 8 g/dl
  • have a history of chronic digestive disease
  • have any gastrointestinal contraindication to ingestion of a capsule (known or suspected gastrointestinal obstruction, stricture, fistula, gastroparesis, or any swallowing disorder)
  • have known immunocompromised status (known history of HIV infection, autoimmune disease, diabetes mellitus, etc.)
  • have known drug hypersensitivity/allergy/intolerance
  • have chronic disease or any other illness or condition which in the opinion of the investigator will complicate the assessment of safety or efficacy
  • are medically disqualified: Any potential participant who is deemed medically unfit for trial enrollment by a non-study healthcare provider, due to the presence of severe or unstable health conditions that could compromise safety or interfere with the study outcomes, will be excluded from participation
  • have a plan to observe fast any time during the intervention period
  • have a plan to leave the study area within the follow-up period
  • are participating in any other interventional trial
  • belong to a household from which another woman is already enrolled in the study
  • but may be enrolled if/when these disqualifiers have expired.

Exclusion

    Key Trial Info

    Start Date :

    August 30 2025

    Trial Type :

    INTERVENTIONAL

    Allocation :

    ESTIMATED

    End Date :

    December 31 2026

    Estimated Enrollment :

    144 Patients enrolled

    Trial Details

    Trial ID

    NCT07207434

    Start Date

    August 30 2025

    End Date

    December 31 2026

    Last Update

    October 6 2025

    Active Locations (1)

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    Mother and Child Health Research and Training Center, AKU

    Matiari, Sindh, Pakistan, 71000