Actively Recruiting

Phase 1
Age: 18Years +
All Genders
NCT07169487

Adjunctive Methylene Blue for Immunotherapy-related CRS and ICANS: Phase I Study

Led by Institute of Hematology & Blood Diseases Hospital, China · Updated on 2025-09-11

18

Participants Needed

1

Research Sites

260 weeks

Total Duration

On this page

AI-Summary

What this Trial Is About

This Phase I, prospective, single-arm clinical study aims to evaluate the efficacy and safety of adjunctive methylene blue (MB) in patients experiencing cytokine release syndrome (CRS) or immune effector cell-associated neurotoxicity syndrome (ICANS) following CAR-T cell therapy or bispecific antibody treatment. Preclinical studies demonstrated that MB alleviates CRS/ICANS-related symptoms, preserves the antitumor function of T cells, and modulates neuroinflammation without compromising immune efficacy. The study will employ a 3+3 dose-escalation design with three MB dosing cohorts, with treatment administered intravenously for 3-5 consecutive days. Vital signs, laboratory markers, and neurological status will be closely monitored, and concomitant standard supportive therapies will be permitted.

CONDITIONS

Official Title

Adjunctive Methylene Blue for Immunotherapy-related CRS and ICANS: Phase I Study

Who Can Participate

Age: 18Years +
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Diagnosed with hematologic malignancies confirmed by cytomorphology and immunophenotyping; age 18 years or older
  • Received immunotherapy such as CAR-T cells or bispecific antibodies and developed Grade 1 or higher CRS or ICANS
  • Have an expected life expectancy of 3 months or more
  • Male and female participants of childbearing potential agree to use effective contraception
  • Left ventricular ejection fraction (LVEF) greater than 45% by echocardiography
  • Ability to understand and sign informed consent and willingness to follow study requirements
Not Eligible

You will not qualify if you...

  • Glucose-6-phosphate dehydrogenase (G6PD) deficiency
  • Known allergy to methylene blue
  • Pregnant or breastfeeding women
  • Known HIV positive status; testing may be required based on local regulations
  • History of serious ventricular arrhythmia, unexplained syncope (not vasovagal), sinoatrial block, or higher-degree AV block with chronic slow heart rate unless a permanent pacemaker is implanted
  • Psychiatric conditions that could interfere with study treatment or consent
  • Any other condition the investigator judges makes participation unsuitable

AI-Screening

AI-Powered Screening

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Trial Site Locations

Total: 1 location

1

Institute of Hematology & Blood Diseases Hospital

Tianjin, Tianjin Municipality, China

Actively Recruiting

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Research Team

J

Jianxiang Wang

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NA

Model

SINGLE_GROUP

Primary Purpose

TREATMENT

Number of Arms

1

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