Study on Lung Immune Cell Response After Lipopolysaccharide Exposure in Adults Aged 18 to 50

What this Trial Is About

This research investigates the role and behavior of different types of alveolar macrophages in lung inflammation and repair, particularly after exposure to endotoxin. The study focuses on understanding how resident alveolar macrophages and recruited macrophages function independently yet work together during lung injury and healing. Researchers aim to better comprehend the cellular processes involved in conditions such as acute respiratory distress syndrome ARDS. The study involves administering lipopolysaccharide LPS, a substance that triggers inflammation, directly into the lungs of healthy adult volunteers through bronchoscopy. Participants then undergo bronchoalveolar lavage BAL at various time points24, 48, 72, 96, or 120 hours after LPS exposureto collect macrophages for analysis. This allows researchers to distinguish and count the different macrophage populations using advanced flow cytometry techniques. Participants will be closely monitored throughout the study period, which includes multiple bronchoscopy procedures and lung fluid collection. Researchers will assess the proportions of resident versus recruited macrophages seven days after LPS administration. Volunteers will undergo medical evaluations including spirometry and electrocardiograms to ensure safety. The study includes healthy adults aged 18 to 50, with continuous monitoring to understand immune responses following controlled lung inflammation.

Alveolar Macrophage Programming Following Endotoxin Exposure