Actively Recruiting
Alveolar Macrophage Programming Following Endotoxin Exposure
Led by National Jewish Health · Updated on 2025-05-06
25
Participants Needed
1
Research Sites
156 weeks
Total Duration
On this page
AI-Summary
What this Trial Is About
This research investigates the role and behavior of different types of alveolar macrophages in lung inflammation and repair, particularly after exposure to endotoxin. The study focuses on understanding how resident alveolar macrophages and recruited macrophages function independently yet work together during lung injury and healing. Researchers aim to better comprehend the cellular processes involved in conditions such as acute respiratory distress syndrome (ARDS). The study involves administering lipopolysaccharide (LPS), a substance that triggers inflammation, directly into the lungs of healthy adult volunteers through bronchoscopy. Participants then undergo bronchoalveolar lavage (BAL) at various time points—24, 48, 72, 96, or 120 hours after LPS exposure—to collect macrophages for analysis. This allows researchers to distinguish and count the different macrophage populations using advanced flow cytometry techniques. Participants will be closely monitored throughout the study period, which includes multiple bronchoscopy procedures and lung fluid collection. Researchers will assess the proportions of resident versus recruited macrophages seven days after LPS administration. Volunteers will undergo medical evaluations including spirometry and electrocardiograms to ensure safety. The study includes healthy adults aged 18 to 50, with continuous monitoring to understand immune responses following controlled lung inflammation.
CONDITIONS
Brief Title
Alveolar Macrophage Programming Following Endotoxin Exposure
Who Can Participate
Eligibility Criteria
You may qualify if you...
- Written, informed consent
- Age 18 to 50 years
You will not qualify if you...
- Current or recent illness within the past 2 weeks
- History of cardiac, pulmonary, or systemic disease
- Bleeding disorder or use of blood thinners
- American Society of Anesthesiology (ASA) class 2 or greater
- Immunocompromised state including HIV or immunosuppressant use
- Use of inhaled substances or smoking history over 10 pack-years
- Alcohol use disorder or excessive drinking in the past 3 months
- Allergy or prior adverse reaction to lidocaine, midazolam, or fentanyl
- Abnormal lung function or heart tests at screening
- Pregnant or breastfeeding women based on urine test
AI-Screening
AI-Powered Screening
Complete this quick 3-step screening to check your eligibility
Your Study Journey
Duration - 2 to 4 weeks
Participants are screened for eligibility to participate in the trial.
Duration - 5 days
Participants will undergo bronchoscopy with intrabronchial administration of lipopolysaccharide followed by bronchoalveolar lavage (BAL) to obtain macrophages at multiple time points.
1 bronchoscopy visit and up to 5 BAL visits over 5 days
Trial Site Locations
Total: 1 location
1
National Jewish Health
Denver, Colorado, United States, 80206
Actively Recruiting
Research Team
K
Kara Mould
How is the study designed?
Study Type
INTERVENTIONAL
Masking
NONE
Allocation
NA
Model
SINGLE_GROUP
Primary Purpose
BASIC_SCIENCE
Number of Arms
1
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