Actively Recruiting

Phase 3
Age: 18Years - 80Years
All Genders
ID06704893

The Efficacy of Amniotic Suspension Allograft Injection in the Treatment of Knee Osteoarthritis: a Randomized Double-blind Controlled Trial

Led by Chang Gung Memorial Hospital · Updated on 2026-01-26

160

Participants Needed

2

Research Sites

N/A

Total Duration

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AI-Summary

What this Trial Is About

Researchers are evaluating the effects of amniotic suspension allograft (ASA) injections compared to hyaluronic acid (HA) and saline injections for treating knee osteoarthritis in adults aged 18 to 80. This condition involves joint cartilage damage and inflammation leading to pain and decreased mobility. The study aims to find the best ASA dose and assess its potential to reduce inflammation and improve quality of life compared to existing injection treatments. Participants will be randomly assigned to receive one of four intra-articular knee injections under ultrasound guidance: ASA 40 mg, ASA 20 mg, HA, or saline. ASA injections are prepared by diluting AMNIOGEN® tissue in saline, while HA and saline are administered as per standard concentrations. The trial is double-blind, ensuring neither participants nor researchers know the assigned treatment. Injections and follow-up occur over a 12-month period. During the study, participants will undergo assessments including pain scales (VAS, NRS), knee injury and osteoarthritis outcome scores (KOOS), and quality of life evaluations (WHOQOL-BREF). Laboratory tests such as complete blood count, C-reactive protein, erythrocyte sedimentation rate, and cytokine levels will be monitored. Imaging with knee ultrasound and X-ray will assess joint status, alongside cartilage protein measurements. Safety and treatment effects will be observed throughout the 12 months of follow-up.

CONDITIONS

Brief Title

Amniotic Suspension Allograft Injection for Knee Osteoarthritis

Who Can Participate

Age: 18Years - 80Years
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Age between 18 and 80 years
  • Body mass index (BMI) less than 40 kg/m²
  • Symptomatic knee osteoarthritis with X-ray evidence graded 2 or 3 on the Kellgren-Lawrence grading scale
  • Numerical Rating Scale (NRS) pain score greater than 4 over the past 7 days
  • Able to walk independently or with the aid of a cane
Not Eligible

You will not qualify if you...

  • Pregnant women or women planning pregnancy
  • Regular use of anticoagulants
  • Use of pain medications (except acetaminophen) within 15 days prior to injection
  • History of substance abuse
  • Use of additional knee symptom-relief medications during the study without reporting to the research team
  • Corticosteroid or visco-supplementation knee injection within 3 months prior to enrollment
  • Knee surgery on the affected side within 12 months before enrollment or surgery on the opposite knee within 6 months before enrollment
  • History of organ or hematopoietic stem cell transplant
  • Current use of immunosuppressive agents
  • Diagnosis of cancer within the past 5 years (except treated basal cell carcinoma)

AI-Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person)

Treatment

Duration - Single treatment visit with follow-up assessments up to 12 months

Participants receive intra-articular knee injections under ultrasound guidance with one of the study drugs: AMNIOGEN® 40mg, AMNIOGEN® 20mg, HyLink (HA), or sterile normal saline.

1 injection visit and multiple follow-up visits over 12 months

Follow-up

Duration - Up to 12 months

Participants are monitored for treatment effects, safety, and quality of life over 12 months after injection.

Multiple follow-up visits over 12 months

Trial Site Locations

Total: 2 locations

1

Kaohsiung Chang Gung Memorial Hospital

Kaohsiung, Taiwan, Taiwan, 833

Actively Recruiting

2

Department of Physical Medicine and Rehabilitation, Kaohsiung Chang Gung Memorial Hospital

Kaohsiung City, Taiwan, 833

Actively Recruiting

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How is the study designed?

Study Type

INTERVENTIONAL

Masking

DOUBLE

Allocation

RANDOMIZED

Model

PARALLEL

Primary Purpose

TREATMENT

Number of Arms

4

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