Actively Recruiting

Phase 1
Age: 18Years +
All Genders
NCT06186401

Anti-EGFRvIII synNotch Receptor Induced Anti-EphA2/IL-13Ralpha2 CAR (E-SYNC) T Cells

Led by Hideho Okada, MD, PhD · Updated on 2026-03-16

20

Participants Needed

1

Research Sites

191 weeks

Total Duration

On this page

Sponsors

H

Hideho Okada, MD, PhD

Lead Sponsor

C

California Institute for Regenerative Medicine (CIRM)

Collaborating Sponsor

AI-Summary

What this Trial Is About

This phase I trial tests the safety, side effects, and best dose of E-SYNC chimeric antigen receptor (CAR) T cells after lymphodepleting chemotherapy in treating patients with EGFRvIII positive (+) glioblastoma. Chimeric antigen receptor (CAR) T-cell therapy is a type of treatment in which a patient's T cells (a type of immune system cell) are changed in the laboratory so the CAR T cells will attack cancer cells. T cells are taken from a patient's blood. Then the gene for a special receptor that binds to a certain protein on the patient's cancer cells is added to the T cells in the laboratory. The special receptor is called a chimeric antigen receptor. Large numbers of the CAR T cells are grown in the laboratory and given to the patient by infusion for treatment of certain cancers. Lymphodepleting chemotherapy with cyclophosphamide and fludarabine before treatment with CAR T cells may make the CAR T cells more effective.

CONDITIONS

Official Title

Anti-EGFRvIII synNotch Receptor Induced Anti-EphA2/IL-13Ralpha2 CAR (E-SYNC) T Cells

Who Can Participate

Age: 18Years +
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Age 18 years or older
  • Karnofsky performance status (KPS) score of 70 or higher
  • Adequate organ function including neutrophil count ≥1000/mm³, platelet count ≥100,000/mm³ without recent transfusions, lymphocyte count ≥300/µL or CD3 count ≥150/µL
  • Creatinine clearance or glomerular filtration rate ≥50 mL/min/1.73m²
  • Total bilirubin ≤1.5 times upper limit of normal (ULN) except for Gilbert's syndrome
  • Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤3 times ULN
  • Left ventricular ejection fraction (LVEF) ≥50% by echocardiogram or MUGA scan
  • Adequate lung function with no resting dyspnea and oxygen saturation >92% on room air
  • Confirmed EGFRvIII positive glioblastoma by certified lab testing
  • MGMT promoter unmethylated or methylation index less than 3
  • Completion of standard external beam radiotherapy as initial therapy
  • Ability to complete E-SYNC T cell infusion within 12 weeks after radiotherapy
  • Off systemic steroids for at least 3 days before leukapheresis
  • Willingness to provide informed consent for apheresis, tissue screening if needed, and study treatment
Not Eligible

You will not qualify if you...

  • Treatment with investigational agents or chemotherapy targeting glioblastoma within 4 weeks before study registration, with specific exceptions for temozolomide and nitrosourea
  • Female participants who are pregnant or breastfeeding
  • Known addiction to alcohol or illicit drugs
  • Prior treatment with any EGFR-targeting therapy
  • Presence of leptomeningeal dissemination
  • Known immune system disorders such as HIV or autoimmune diseases requiring systemic cytotoxic or immunosuppressive therapy
  • Positive hepatitis B surface antigen or active hepatitis B or C infection confirmed by PCR
  • Prior solid organ or bone marrow transplant
  • Uncontrolled illnesses including active infection, heart failure, unstable angina, arrhythmia, active second cancers, or psychiatric/social conditions limiting study compliance
  • Inability to return for follow-up visits or complete toxicity assessments

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Trial Site Locations

Total: 1 location

1

University of California, San Francisco

San Francisco, California, United States, 94143

Actively Recruiting

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Research Team

N

Neuro-Oncology New Patient Coordinator

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NON_RANDOMIZED

Model

SEQUENTIAL

Primary Purpose

TREATMENT

Number of Arms

3

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