Study of Gene Expression in Egg Cells of Women Aged 18 to 35 Using Hormone Stimulation for Egg Donation

What this Trial Is About

Researchers are conducting a randomized observational study to explore gene expression profiles in cumulus cells surrounding oocytes from women undergoing ovarian stimulation. The goal is to identify mRNA markers that predict oocyte competence and potential for forming high-quality blastocysts and achieving clinical pregnancy. The study involves two groups of women treated with different ovarian stimulation protocols using human recombinant FSH and GnRH agonist triggers in an egg donation program. Participants are divided into two cohorts one receiving GnRH antagonist with hr-FSH and GnRH agonist trigger, and the other receiving progesterone with hr-FSH and GnRH agonist trigger. The study observes gene expression in cumulus cells collected from oocytes after denudation during egg retrieval. This includes an examination of basic stimulation characteristics such as hormone levels, follicle counts, fertilization rates, and embryo quality up to day 6 of embryo culture. During the study, participants undergo ovarian stimulation for up to three weeks with monitoring of hormones like progesterone and estradiol, follicle development, and oocyte maturity. Researchers assess fertilization outcomes and embryo development across several days. The study also measures gene expression profiles and pregnancy outcomes up to 2.5 years after starting. Patient consent for oocyte testing and detailed clinical evaluations are part of the process, with ongoing data collection on pregnancy and blastocyst formation.

Aurora Test for ART Donor Patients (AURORA-Donor)