Actively Recruiting

Phase 1
Phase 2
Age: 2Months +
All Genders
NCT03538899

Autologous Gene Therapy for Artemis-Deficient SCID

Led by University of California, San Francisco · Updated on 2026-02-13

24

Participants Needed

1

Research Sites

1043 weeks

Total Duration

On this page

AI-Summary

What this Trial Is About

This study aims to determine if a new method can be used to treat Artemis-deficient Severe Combined Immunodeficiency (ART-SCID), a severe form of primary immunodeficiency caused by mutations in the DCLRE1C gene. This method involves transferring a normal copy of the DCLRE1C gene into stem cells of an affected patient. Participants will receive an infusion of stem cells transduced with a self-inactivating lentiviral vector that contains a normal copy of the DCLRE1C gene. Prior to the infusion they will receive sub-ablative, dose-targeted busulfan conditioning. The study will investigate if the procedure is safe, whether it can be done according to the methods described in the protocol, and whether the procedure will provide a normal immune system for the patient. A total of 24 newly diagnosed patients will be enrolled at the University of California San Francisco in this single-site trial and will be followed for 15 years post-infusion. It is hoped that this type of gene transfer may offer improved outcomes for ART-SCID patients who lack a brother or sister who can be used as a donor for stem cell transplantation or who have failed to develop a functioning immune system after a previous stem cell transplant.

CONDITIONS

Official Title

Autologous Gene Therapy for Artemis-Deficient SCID

Who Can Participate

Age: 2Months +
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • At least 2.0 months old at start of busulfan conditioning
  • Newly diagnosed with typical or minimally leaky ART-SCID defined by bi-allelic pathogenic mutations in DCLRE1C
  • CD3 count less than 50 autologous cells/µL or evidence of maternal chimerism, or CD3 count 50-300/µL with restricted T cell receptor diversity
  • CD45 cell response to mitogens less than 50% of the lower limit of normal (for leaky ART-SCID)
  • No medically eligible HLA-identical sibling with normal immune system available as donor (for newly diagnosed patients)
Not Eligible

You will not qualify if you...

  • Presence of a medically eligible HLA-matched sibling
  • Evidence of HIV infection by PCR or p24 antigen test
  • Unable to tolerate general anesthesia or marrow harvest or central venous catheter insertion
  • Liver function tests (AST, ALT, GGT) more than 5 times upper limit of normal or total bilirubin over 2.0 mg/dl (unless due to specific exceptions) at start of busulfan conditioning
  • Severe medical conditions making busulfan administration unsafe
  • Recognized second gene mutation causing a hematopoietic disorder treatable by allogeneic transplant
  • Medical condition predicting survival less than 4 months, such as need for mechanical ventilation or severe organ failure
  • Family situation that may prevent following protocol or medical care
  • Other conditions deemed by investigators to contraindicate infusion or study participation

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Trial Site Locations

Total: 1 location

1

University of California, San Francisco (UCSF) Children's Hospital

San Francisco, California, United States, 94143

Actively Recruiting

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Research Team

M

Morton Cowan, MD

CONTACT

J

Jennifer Puck, MD

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NA

Model

SINGLE_GROUP

Primary Purpose

TREATMENT

Number of Arms

1

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