Actively Recruiting

Phase 1
Phase 2
Age: 18Years - 75Years
All Genders
ID07123545

Feasibility, Safety and Efficacy Study of Autologous Neoantigen-Specific T-Cell Therapy in Advanced Hepatocellular Carcinoma Patients

Led by Zhejiang University · Updated on 2025-08-14

20

Participants Needed

1

Research Sites

104 weeks

Total Duration

On this page

Sponsors

Z

Zhejiang University

Lead Sponsor

H

Hangzhou Neoantigen Therapeutics Co., Ltd.

Collaborating Sponsor

AI-Summary

What this Trial Is About

Researchers are evaluating an autologous neoantigen-specific T-cell therapy called iNeo-Vac-T01 for patients with advanced hepatocellular carcinoma who have not responded to two or more prior systemic treatments. This open-label, single-arm phase I/II trial aims to assess the feasibility, safety, and anti-tumor effects of this personalized immunotherapy approach in this patient population. The study is sponsored by Zhejiang University and focuses on advanced cancer that is measurable by imaging and has progressed despite previous therapies. Participants receive a personalized neoantigen peptide vaccine (iNeo-Vac-P01) given by subcutaneous injection on Days 1, 4, 8, 15, 22, 52, and 82, followed by booster shots every 2 to 3 months. Starting at Week 9, patients undergo intravenous infusion of the personalized T cell therapy (iNeo-Vac-T01) using a dose escalation design. Before this infusion, a lymphodepleting regimen with cyclophosphamide and fludarabine is administered. After infusion, supportive therapy with interleukin-2 is given for 10 days to support immune response. During the study, participants will have regular visits for treatment administration and monitoring for up to three years. Researchers will evaluate the success of administering iNeo-Vac-T01 and monitor for adverse events. Secondary measures include progression-free survival, tumor response rates, and immune response in the blood. Patients will undergo laboratory tests, imaging scans, and genomic profiling of tumor tissue to support treatment planning and safety assessments. The total participation duration may last several years as patients are followed for clinical outcomes and safety.

CONDITIONS

Brief Title

Autologous Neoantigen-Specific T-Cell Therapy for Advanced Hepatocellular Carcinoma

Who Can Participate

Age: 18Years - 75Years
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Aged 18 to 75 years (inclusive)
  • Histologically confirmed advanced hepatocellular carcinoma with measurable disease per RECIST v1.1
  • Documented progression on two or more prior lines of systemic therapy
  • Life expectancy of at least 6 months
  • ECOG performance status of 0 or 1
  • Available tumor tissue or genomic sequencing data suitable for analysis
  • Adequate hematologic, hepatic, renal, and coagulation function
  • Women of childbearing potential must have negative pregnancy test and agree to use effective contraception
  • Men must agree to use barrier contraception
  • Willing and able to comply with study visits and procedures
Not Eligible

You will not qualify if you...

  • History of other active cancers within 5 years except certain treated skin or thyroid cancers
  • Inability to identify targetable neoantigens from genomic analysis
  • Prior allogeneic bone marrow, solid organ, or stem cell transplantation
  • Active or symptomatic central nervous system metastases, except stable treated cases
  • Active infections requiring systemic therapy, including untreated latent tuberculosis
  • Active viral infections such as hepatitis B, hepatitis C, HIV, or syphilis
  • Active autoimmune disease requiring significant immunosuppression, except specified exceptions
  • Recent systemic immunosuppressive therapy within 14 days prior to cell infusion
  • Uncontrolled serious medical conditions including severe heart failure, unstable angina, severe arrhythmias, uncontrolled hypertension, or significant lung disease
  • History of substance abuse or psychiatric conditions impairing consent or compliance
  • History of severe allergic reactions to vaccines or study products
  • Any condition posing unacceptable risk or interfering with study participation per investigator judgment

AI-Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person)

Treatment

Duration - Approximately 3 months for initial vaccination and cell infusion, followed by booster immunizations every 2 to 3 months

Participants receive a personalized neoantigen peptide vaccine administered subcutaneously on Days 1, 4, 8, 15, 22, 52, and 82, followed by booster immunizations every 2 to 3 months. After initial vaccination, participants receive personalized T cell injections via intravenous infusion starting at Week 9, preceded by lymphodepleting preconditioning, and followed by 10 days of supportive therapy.

Multiple visits including 7 vaccination visits in the first 12 weeks, cell infusion at Week 9, and supportive therapy visits

Follow-up

Duration - Up to 3 years

Participants are monitored for safety, efficacy, and immune response after completing treatment.

Periodic visits for up to 3 years

Trial Site Locations

Total: 1 location

1

The First Affiliated Hospital, Zhejiang University School of Medicine

Hangzhou, Zhejiang, China

Actively Recruiting

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Research Team

T

Tingbo Liang

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NA

Model

SINGLE_GROUP

Primary Purpose

TREATMENT

Number of Arms

1

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