Actively Recruiting

Age: 18Years +
All Genders
Healthy Volunteers
NCT04095156

Autoreactive B Cells in Membranous Nephropathy

Led by Mario Negri Institute for Pharmacological Research · Updated on 2026-03-20

86

Participants Needed

1

Research Sites

370 weeks

Total Duration

On this page

AI-Summary

What this Trial Is About

Membranous nephropathy (MN) is the most frequent cause of nephrotic syndrome (NS) in adults. The majority of MN patients show detectable circulating antibodies against the M-type phospholipase A2 receptor (PLA2R). Infusion of anti-CD20 monoclonal antibodies results in a profound depletion of B-cells, which are thought to be responsible for anti-PLA2R production. B-cell depletion is followed by NS remission in 70% of cases. Limited evidence highlighted that differences in the B- and T-cell compartments may exist between responders and non-responders. Owing to the non-homogenous efficacy of anti-CD20 treatment, investigators hypothesize that in MN patients who experience NS remission after B-cell depleting therapy, autoreactive B-cells may be mostly circulating, whereas in patients who do not respond to the same treatment, autoreactive B-cells may chiefly reside into secondary lymphoid organs - and thus be more resistant to the drug action. Researchers will therefore extensively analyze the circulating immune repertoire of MN patients before and after the infusion of B-cell lineage depleting agents, assessing the presence of circulating PLA2R autoreactive B cells from appropriately stratified responder and non-responder patients. Patients and healthy controls will be enrolled in this study. Patients will be stratified according to gender, anti-PLA2R status, type of B-cell lineage depleting agent received and response to treatment.

CONDITIONS

Official Title

Autoreactive B Cells in Membranous Nephropathy

Who Can Participate

Age: 18Years +
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Male or female adults over 18 years old
  • Patients with biopsy-proven idiopathic membranous nephropathy who are candidates for or have received B-cell depleting treatment
  • Mental ability to understand and give valid consent
  • Written informed consent according to the Declaration of Helsinki
  • Healthy volunteers over 18 years old without significant illness
  • Healthy volunteers not taking regular medications
  • Healthy volunteers with negative urine analysis
  • Written informed consent for healthy volunteers according to the Declaration of Helsinki
Not Eligible

You will not qualify if you...

  • Possibility of secondary causes of membranous nephropathy such as systemic lupus erythematosus, active hepatitis B, malignancy, or certain drugs
  • Legal incapacity, intellectual disability, dementia, uncooperative attitude, or inability to give informed consent

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Trial Site Locations

Total: 1 location

1

Centro di Ricerche Cliniche per le Malattie Rare " Aldo e Cele Daccò"

Ranica, BG, Italy, 24020

Actively Recruiting

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Research Team

M

Manuel Podestà, MD

CONTACT

How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

3

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