Actively Recruiting

Phase 1
Age: 18Years +
All Genders
ID05945849

Phase 1 Study of Lentivirally Transduced T Cells Engineered to Contain Anti-CD33 Linked to TCRζ And 4-1BB Signaling Domains Combined With CD33KO-HSPC in Refractory or Relapsed Acute Myeloid Leukemia

Led by University of Pennsylvania · Updated on 2025-06-29

16

Participants Needed

1

Research Sites

782 weeks

Total Duration

On this page

AI-Summary

What this Trial Is About

Researchers are evaluating a new treatment approach for adults with refractory or relapsed acute myeloid leukemia (AML). This phase 1 study explores combining a modified stem cell transplant with engineered chimeric antigen receptor (CAR) T cells designed to recognize and attack AML cells. The modified transplant makes healthy bone marrow cells "invisible" to the CAR T cells to improve safety during treatment. Participants receive a stem cell transplant using donor blood-forming stem cells that have been gene-edited to remove a protein called CD33. After the transplanted stem cells begin producing healthy blood cells, patients receive one to three infusions of CAR T cells from the same donor. These CAR T cells are engineered to target CD33, a protein found on leukemia cells and healthy bone marrow cells. During the study, participants will be monitored for manufacturing feasibility and dose-limiting toxicities over several months. Researchers will assess treatment safety, efficacy, overall and progression-free survival, and duration of response up to 15 years. Assessments include regular clinical evaluations, laboratory tests, and imaging to track health, treatment effects, and leukemia progression. Participants will be followed closely throughout the study and beyond for up to 20 years.

CONDITIONS

Brief Title

CD33KO-HSPC Infusion Followed by CART-33 Infusion(s) for Refractory/Relapsed AML

Who Can Participate

Age: 18Years +
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Male or female 18 years of age or older
  • Diagnosis of AML unlikely to be cured with current therapies
  • AML not in complete remission or with relapsed disease after transplant
  • Off systemic immunosuppression for at least 1 month if relapsed after prior transplant
  • Suitable stem cell donor available
  • Creatinine clearance greater than 40 ml/min
  • ALT/AST less than or equal to 5 times upper limit of normal unless disease-related
  • Direct bilirubin less than 2.0 mg/dl (or less than or equal to 3.0 mg/dl with Gilbert's syndrome)
  • Left ventricular ejection fraction 40% or higher
  • DLCO greater than 45% predicted
  • ECOG performance status 0 or 1
  • Written informed consent provided
  • Agreement to use acceptable birth control if of reproductive potential
Not Eligible

You will not qualify if you...

  • Pregnant or lactating women
  • Active hepatitis B, hepatitis C, or HIV infection
  • Concurrent use of systemic steroids or immunosuppressant medications
  • Uncontrolled active medical disorders that preclude participation
  • Signs or symptoms of CNS leukemia involvement
  • Known allergy or hypersensitivity to study product excipients
  • Class III or IV cardiovascular disability by New York Heart Association Classification
  • History of optic neuritis or other central nervous system inflammatory diseases unrelated to leukemia
  • Clinically apparent or unstable arrhythmias within 2 weeks before enrollment

AI-Screening

AI-Powered Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

Treatment

Duration - Up to 6 months

Participants receive a stem cell transplant with gene-edited cells to make the therapy safer, followed by 1 to 3 infusions of CAR T-cells designed to target leukemia cells.

Trial Site Locations

Total: 1 location

1

University of Pennsylvania

Philadelphia, Pennsylvania, United States, 19104

Actively Recruiting

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Research Team

A

Abramson Cancer Center Clinical Trials Service

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NA

Model

SINGLE_GROUP

Primary Purpose

TREATMENT

Number of Arms

1

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