Actively Recruiting

Age: 18Years - 45Years
FEMALE
ID06788379

CENP-V As a Potential Diagnostic Marker of Damage in Human Oocytes

Led by Vida Recoletas Sevilla · Updated on 2025-01-23

160

Participants Needed

1

Research Sites

26 weeks

Total Duration

On this page

Sponsors

V

Vida Recoletas Sevilla

Lead Sponsor

U

University of Seville

Collaborating Sponsor

AI-Summary

What this Trial Is About

Researchers are investigating the levels of the CENP-V protein in human oocytes to understand its role as a potential diagnostic marker for oocyte damage related to aging and the freezing process called vitrification. The study focuses on women with advanced maternal age (AMA) and compares them to younger control patients. The research addresses a concern that aging and freezing may reduce oocyte quality, affecting fertility and survival rates after thawing. The study involves analyzing immature oocytes that would otherwise be discarded from patients with AMA and control patients, as well as oocytes subjected to vitrification and thawing. Patients are divided into groups by age and fertility status, including those undergoing ovarian stimulation and oocyte collection. Only immature or donated mature oocytes not used for treatment are included in the analysis. Participants will have their oocytes collected and assessed within two days from enrollment to retrieval. The study measures the levels and distribution of CENP-V in these oocytes and tracks the survival rate of oocyte cohorts after thawing. This observational study does not involve treatment but focuses on laboratory analysis to identify markers of oocyte damage, which could inform future fertility evaluations.

CONDITIONS

Brief Title

CENP-V As a Potential Diagnostic Marker of Damage in Human Oocytes

Who Can Participate

Age: 18Years - 45Years
FEMALE

Eligibility Criteria

Eligible

You may qualify if you...

  • Patients aged 36 to 45 years with advanced maternal age as the main cause of infertility undergoing controlled ovarian stimulation
  • Patients aged 18 to 45 years who have undergone oocyte vitrification and thawing, including subgroups of advanced maternal age (36-45 years) and non-advanced maternal age (18-35 years)
  • Control patients and donors aged 18 to 35 years with normal fertility undergoing ovarian stimulation and oocyte collection
Not Eligible

You will not qualify if you...

  • Patients with conditions affecting oocyte quality such as polycystic ovary syndrome, premature ovarian insufficiency, endometriosis, or cancer
  • Patients or donors whose oocytes were cryopreserved using slow freezing techniques rather than vitrification

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person)

Sample Collection

Duration - 2 days

Participants undergo ovarian stimulation and oocyte retrieval, during which immature or donated mature oocytes are collected for analysis.

1 to 2 visits depending on ovarian stimulation and retrieval schedule

Laboratory Analysis

Duration - Varies depending on sample processing

Collected oocytes are analyzed fresh and after vitrification-thawing to measure CENP-V protein levels as a diagnostic marker of damage.

No participant visits; analysis performed on collected samples

Trial Site Locations

Total: 1 location

1

Vida Recoletas Sevilla S.L.

Seville, SEVILLA, Spain, 41009

Actively Recruiting

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Research Team

E

ESTHER SANTAMARÍA LÓPEZ

How is the study designed?

Study Type

OBSERVATIONAL

Masking

N/A

Allocation

N/A

Model

N/A

Primary Purpose

N/A

Number of Arms

2

Frequently Asked Questions

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