Evaluation of the Antibacterial Efficacy of Chitosan as an Irrigation Solution: A Randomized Controlled Clinical Trial

What this Trial Is About

Researchers are evaluating which of three common root-canal rinsing methods best reduces bacteria in adults needing root-canal treatment. The study focuses on whether chitosan alone, sodium hypochlorite combined with chitosan, or sodium hypochlorite combined with EDTA achieves the greatest bacterial reduction inside the root canal. This randomized controlled trial aims to provide clearer information on effective irrigation solutions for dental infections like apical periodontitis and root canal infection. Participants are randomly assigned to one of three groups: chitosan irrigation only; sodium hypochlorite followed by chitosan; or sodium hypochlorite followed by EDTA. Each solution is delivered into the root canal with specific contact times and followed by a sterile saline flush. Treatment takes place over two clinic visits: the first involves standard root-canal therapy with sample collection before and after rinsing, and the second visit, about a week later, is for a check-up and final tooth filling. Participants will provide tiny samples from inside the root canal for bacterial measurement before and immediately after treatment. They will also record any pain or discomfort for three days post-treatment using a diary or phone calls. The main outcome measured is the change in bacterial load inside the canal from before to after treatment. The study lasts about one week, involves approximately 90 adults, and all procedures are routine dental care provided at no cost. Participants may withdraw at any time and can ask questions throughout the trial.

Chitosan Irrigation in Premolar Root Canal Therapy (Randomized Trial)