Actively Recruiting

Phase 1
Age: 18Years - 75Years
All Genders
ID05410717

Phase I Trial to Evaluate Safety and Preliminary Efficacy of CLDN6/GPC3/Mesothelin/AXL-CAR-NK in Patients With CLDN6/GPC3/Mesothelin/AXL-positive Advanced Solid Tumors

Led by Second Affiliated Hospital of Guangzhou Medical University · Updated on 2024-06-25

200

Participants Needed

1

Research Sites

313 weeks

Total Duration

On this page

AI-Summary

What this Trial Is About

This research aims to evaluate the safety and early effectiveness of CAR-NK cell therapies targeting Claudin6, GPC3, Mesothelin, or AXL in patients with advanced solid tumors, including ovarian cancer. The study focuses on patients whose tumors express these specific markers and explores different genetic modifications to enhance the anti-cancer activity of the CAR-NK cells. This is an open, exploratory Phase 1 clinical trial conducted by the Second Affiliated Hospital of Guangzhou Medical University. Participants will undergo a procedure to collect their natural killer (NK) cells, which are then engineered to carry CARs targeting Claudin6, GPC3, Mesothelin, or AXL. Some CAR-NK cells are further engineered to secrete IL7/CCL19 or scFvs against PD1/CTLA4/Lag3 to boost effectiveness. Others may be combined with cannabidiol (CBD) or nicotinamide adenine dinucleotide (NAD). Patients receive these modified cells via systemic or local infusions, and similar therapies are tested across different cancer types expressing these markers. During the study, patients are closely monitored for safety according to standard criteria for adverse events for up to 52 weeks after infusion. Researchers will also assess tumor response rates, disease control, and duration of response for up to three years. Quality and activity of the infused CAR-NK cells are tested before administration. Follow-up includes clinical evaluations and laboratory testing to track treatment effects and safety throughout the trial.

CONDITIONS

Brief Title

CLDN6/GPC3/Mesothelin/AXL-CAR-NK Cell Therapy for Advanced Solid Tumors

Who Can Participate

Age: 18Years - 75Years
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Patients with advanced ovarian cancer or other cancers expressing Claudin6
  • Life expectancy greater than 12 weeks
  • Adequate heart, lung, liver, and kidney function
  • Autologous transduced NK cells with at least 20% expression of Claudin6, GPC3, Mesothelin, or AXL-CAR by flow cytometry
  • Cytotoxicity assay showing at least 20% killing of Claudin6-positive targets
  • Signed informed consent by patient or guardian
Not Eligible

You will not qualify if you...

  • Prior acceptance of gene therapy
  • Tumor size larger than 25 cm
  • Severe viral infections such as HBV, HCV, or HIV
  • Known HIV positivity
  • History of liver or kidney transplantation
  • Active infectious diseases related to bacteria, virus, or fungi
  • Other severe diseases deemed inappropriate by investigators
  • Pregnant or lactating women
  • Systemic steroid treatment at or above 0.5 mg prednisone equivalent per kg per day
  • Other conditions considered inappropriate by investigators

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person)

Treatment

Duration - Up to 52 weeks after CAR-NK cell infusion

Participants receive CLDN6, GPC3, Mesothelin, or AXL-targeting CAR-NK cell therapy, which may include genetically engineered cells secreting IL7/CCL19 or scFv against PD1/CTLA4/Lag3, and some may receive additional treatment with Cannabidiol (CBD) or NAD to enhance therapy.

Visits as needed for infusion and monitoring

Follow-up

Duration - Up to 36 months

Participants are closely followed to monitor safety, response, and disease control after CAR-NK cell infusion, including assessments up to 36 months for duration of response.

Visits as needed for follow-up assessments

Trial Site Locations

Total: 1 location

1

The Second Affiliated Hospital of Guangzhou Medical University

Guangzhou, Guangdong, China

Actively Recruiting

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Research Team

Z

Zhenfeng Zhang, MD, PHD

B

Bingjia He, MD

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

RANDOMIZED

Model

PARALLEL

Primary Purpose

TREATMENT

Number of Arms

5

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