Actively Recruiting

Phase 1
Phase 2
Age: 18Years +
All Genders
NCT06961357

Clinical Trial of CD40L-augmented TIL for Patients With Advanced Melanoma

Led by H. Lee Moffitt Cancer Center and Research Institute · Updated on 2026-03-31

36

Participants Needed

1

Research Sites

230 weeks

Total Duration

On this page

AI-Summary

What this Trial Is About

This is a phase I/II clinical trial of a single dose of CD40L-augmented TIL administered in patients with advanced melanoma (Cohort 1: Cutaneous acral melanoma, cutaneous non-acral melanoma, (n=26); Cohort 2: Mucosal melanoma, uveal melanoma, (n=10)). Patients will undergo an excision of a readily accessible tumor for preparation of TIL. Eligible patients with progressive disease after standard of care therapy will undergo lymphodepletion with cyclophosphamide and fludarabine followed by CD40L-augmented TIL and standard of care bolus dose interleukin-2 (short-course IL-2).

CONDITIONS

Official Title

Clinical Trial of CD40L-augmented TIL for Patients With Advanced Melanoma

Who Can Participate

Age: 18Years +
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Histologically confirmed unresectable Stage III/IV or metastatic melanoma including cutaneous non-acral, acral, mucosal, or ocular types
  • Failed, refractory to, or unable to tolerate at least one line of standard of care therapy including PD-1/L1 inhibitors or targeted therapies
  • Completed any systemic therapy at least 4 weeks before lymphodepleting therapy with resolved adverse events to Grade 1 or less except alopecia and vitiligo
  • Age 18 or older; patients 65 or older may require cardiac evaluation to confirm low risk
  • ECOG performance status of 0 or 1
  • Adequate organ and marrow function per protocol
  • Seronegative for HIV antibody, hepatitis B surface antigen, and hepatitis C antibody; if HCV antibody positive, must have negative HCV RNA
  • Brain metastases are allowed if treated and stable for at least 4 weeks
  • Negative pregnancy test for women of childbearing potential
  • Both males and females of childbearing potential must agree to use birth control during the study and for specified periods after last drug administration
  • Able to understand and sign informed consent
  • At least one surgically accessible lesion for tumor harvest and one measurable lesion for response assessment
Not Eligible

You will not qualify if you...

  • History of ischemic heart disease or significant heart rhythm abnormalities without cardiology clearance
  • Primary or acquired immunodeficiency disorders including HIV/AIDS
  • Pregnant or breastfeeding women
  • Current systemic steroid therapy above 10 mg prednisone equivalent or other immunosuppressive medications, except allowed replacement or stress doses
  • History of severe allergic reaction to study drugs including cyclophosphamide, fludarabine, or IL-2
  • Left ventricular ejection fraction 45% or less or NYHA functional class above 1
  • Pulmonary function below specified thresholds unless stable with recent testing
  • Medical conditions posing prohibitive risk for study participation or inability to complete treatments
  • Active infections requiring antibiotics
  • Active autoimmune diseases requiring systemic immunosuppressive treatment
  • Prior live cell therapy without written permission from the principal investigator

AI-Screening

AI-Powered Screening

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Trial Site Locations

Total: 1 location

1

Moffitt Cancer Center

Tampa, Florida, United States, 33612

Actively Recruiting

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Research Team

T

Tina Swartzlander

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NON_RANDOMIZED

Model

SEQUENTIAL

Primary Purpose

TREATMENT

Number of Arms

3

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