Comparing Manual-Less and Conventional Rotary Techniques for Root Canal Treatment Effect on Postoperative Pain and Neuropeptide Levels in Adults 18 to 50 Years

What this Trial Is About

Researchers are evaluating two root canal instrumentation methods to compare their effects on post-operative pain and the levels of certain neuropeptides, Substance P and Calcitonin gene-related peptide CGRP. The study includes 34 permanent molars with symptomatic irreversible pulpitis and apical periodontitis. This research aims to explore how a manual-less technique, which skips manual scouting and glide path steps, compares to a conventional rotary technique in terms of pain and biochemical markers after treatment. Participants will be randomly assigned to one of two groups the manual-less group, using rotary Dota files without scouting and glide path preparation, and the conventional group, which uses rotary Dota files following manual scouting and glide path creation with hand files. Levels of Substance P and CGRP will be measured before and after instrumentation using Elisa analysis. The study does not involve any masking or blinding. Participants will be monitored for post-operative pain at 6, 12, 24, 48, and 72 hours after the procedure. Pain levels will be assessed using a numeric rating scale. The correlation between post-operative pain and neuropeptide levels will also be analyzed, with samples collected immediately after instrumentation and at 72 hours. The study is expected to conclude by March 2026.

Comparison Between Two Instrumentation Techniques on Postoperative Pain and Level of Neuropeptides