Improving Microbiological Diagnosis in Adults with Complex Eye Infections Using Advanced Molecular Techniques

What this Trial Is About

Researchers are studying complex ocular infections, including endophthalmitis and corneal abscesses, to improve the methods used for microbiological diagnosis. These infections are challenging to diagnose because of small sample volumes and difficult access to the eye. New molecular tools like metagenomic sequencing and multiplex polymerase chain reaction PCR are being evaluated to optimize diagnosis accuracy and speed. The study involves patients with complex ocular infections. For endophthalmitis, next-generation sequencing NGS and culture methods will be used to analyze ocular samples. For corneal abscesses, multiplex PCR will be added to the diagnostic process to potentially shorten the time to diagnosis. These molecular techniques are being assessed alongside standard methods to understand their impact on diagnosis. Participants will provide ocular samples which will be tested with the new molecular techniques and traditional culture methods. Researchers will measure the positivity rates of samples within two weeks, and evaluate microbiological diagnosis accuracy, time to diagnosis, visual acuity, cure rates, and any changes in anti-infectious treatment during an 18-month follow-up. The study is observational and aims to improve diagnosis for better management of these infections.

Complex Ocular Infection, Optimization of Microbiological Diagnosis