Actively Recruiting

Phase 1
Phase 2
Age: 16Years - 85Years
All Genders
NCT07053488

CRISPR-Edited HLA Donor Liver Transplant to Reduce Rejection

Led by AMERICAN ORGAN TRANSPLANT AND CANCER RESEARCH INSTITUTE LLC · Updated on 2025-07-08

90

Participants Needed

1

Research Sites

186 weeks

Total Duration

On this page

AI-Summary

What this Trial Is About

This early-phase clinical trial will assess the use of ex vivo CRISPR-Cas9 genome editing on donor liver grafts to reduce immunogenicity before transplantation. Donor livers will have HLA-A and HLA-B genes knocked out, and HLA class II expression disabled (by targeting the CIITA transactivator gene), aiming to create a "hypoimmunogenic" organ less prone to rejection. The edited liver is then transplanted into patients with end-stage liver disease. The primary focus is on safety and feasibility - determining whether a CRISPR-edited liver can be transplanted successfully and function normally - as well as evaluating reductions in immune response (acute rejection, anti-donor T cell activation) and graft function over time.

CONDITIONS

Official Title

CRISPR-Edited HLA Donor Liver Transplant to Reduce Rejection

Who Can Participate

Age: 16Years - 85Years
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Adults aged 16-85 (inclusive) with end-stage liver disease or acute liver failure eligible for liver transplantation
  • Require a liver transplant and have been assigned a donor liver graft from a deceased donor to be used after gene editing
  • No immediately available fully HLA-matched donor
  • Medically suitable for transplant surgery and able to tolerate standard immunosuppressive therapy
  • Able to understand the investigational nature and provide written informed consent
  • Willing to comply with study procedures and available for follow-up including frequent visits
Not Eligible

You will not qualify if you...

  • Active uncontrolled infection such as sepsis or active tuberculosis
  • Uncontrolled HIV or poorly managed chronic viral infections
  • Need for multi-organ transplant beyond liver alone
  • Pregnancy or breastfeeding without effective contraception
  • Severe illness limiting survival to less than 1 year or making transplant unsuitable
  • Known severe allergy to study-related products such as CRISPR components
  • Inability to follow the study protocol or comply with follow-up due to psychiatric, social, or logistical reasons

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Trial Site Locations

Total: 1 location

1

Peking University Health Science Center (PKUHSC)

Beijing, Changping, China, 102206

Actively Recruiting

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Research Team

A

Andrew R Linehan

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NA

Model

SINGLE_GROUP

Primary Purpose

TREATMENT

Number of Arms

1

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