Detecting Azole Resistance Mutations from Blood Samples in Immunocompromised Adults with Invasive Aspergillosis

What this Trial Is About

This research focuses on invasive aspergillosis IA, a common fungal infection affecting immunocompromised patients with blood-related diseases. The study aims to improve diagnosis by detecting mutations in fungal DNA that cause resistance to azole drugs, which are the main treatments for IA. This is especially important in the Netherlands, where resistance is common and linked to high mortality rates. Participants will have Aspergillus PCR tests performed on different volumes of their serum and plasma. These tests seek to quickly identify azole resistance mutations directly from blood samples, bypassing slower fungal cultures. The study evaluates the performance of two PCR tests, compares different media used in these tests, and determines the optimal PCR cycle threshold within one week. During the study, participants undergo lung CT scans and bronchoalveolar lavage as part of their care. Researchers collect blood samples for PCR testing and assess test performance shortly after. The primary outcomes focus on how well the PCR tests detect resistance mutations. Participants must be adults with specific lung lesions and planned or recent lavage. The study excludes those unable or unwilling to provide consent.

Detection of Azole Resistance Inducing Mutations on DNA Extracted Directly From Serum or Plasma of Immunocompromised Patients With an Invasive Aspergillus Infection Azole Resistance PCR Optimalization-study