Pilot Feasibility Study to Develop Organoids from Primary Colorectal Tumors and Liver Metastases To Study Gene Functions Related to the NF-Y/p53 Complex

What this Trial Is About

This research focuses on developing organoids from primary colorectal tumors and synchronous liver metastases. These organoids will be used to study the function of genes targeted by the NF-Y/p53 complex activity. The goal is to better understand how these genes influence cancer cell behavior and to explore new pathways that might be targeted by therapies. Organoids will be prepared from fresh colorectal cancer tissue and liver metastases using enzymatic digestion. They will be cultured in a specific organic matrix with a specially formulated medium. In these organoids, researchers will perform experiments to overexpress or silence target genes and assess cell proliferation, invasion, and movement. Functional assays will also be conducted using co-cultures of the organoids with peripheral blood mononuclear cells from the same patients. Participants will provide tissue samples during surgery for colorectal cancer with liver metastases. The study includes follow-up assessments over 36 months, focusing on descriptive analyses of the organoids and their behavior. Researchers aim to gather detailed information about gene function and potential therapeutic targets through these laboratory tests and long-term monitoring.

Development of Organoids From Primary Colorectal Tumors and Synchronous Liver Metastases