Actively Recruiting

Phase Not Applicable
Age: 18Years - 40Years
All Genders
Healthy Volunteers
ID04122456

Pilot Study on DNA Repair Activity in the Skin of Day and Night Shift Workers

Led by Wright State University · Updated on 2025-02-06

48

Participants Needed

1

Research Sites

N/A

Total Duration

On this page

AI-Summary

What this Trial Is About

Researchers are studying how the skin of night shift workers responds to artificial sunlight, specifically ultraviolet B radiation (UVB), compared to day shift workers. The study aims to understand differences in DNA repair activity and clock gene expression in skin exposed to UVB at two different times of day. This research focuses on skin cancer risk factors related to work schedules and skin response to UVB radiation. The study involves taking skin punch biopsies from both day and night shift workers. These biopsies will be brought to a laboratory where they will be exposed to UVB radiation or kept as non-irradiated controls. After one hour of incubation from UVB exposure, various DNA repair factors, core clock genes, and DNA damage signaling pathways will be measured. Participants will fall into two groups based on their work schedules: those working primarily daytime shifts and those working primarily night shifts. Participants will provide information about their work schedules over the past three months and undergo skin biopsies for laboratory testing. Researchers will evaluate the expression levels of DNA repair proteins, activity of DNA repair systems, and activation of DNA damage signaling after UVB exposure. The study includes assessments at two times of day to compare biological responses. The total study participation involves skin biopsies and data collection related to work schedules and skin type.

CONDITIONS

Brief Title

DNA Repair Activity in the Skin of Day and Night Shift Workers

Who Can Participate

Age: 18Years - 40Years
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Male or female
  • Fair skin (Fitzpatrick types I and II)
  • Age 18 to 40 years
  • Able to understand study procedures and risks
  • Primarily work and stay awake during normal daylight hours (6 am to 6 pm) or primarily during night shifts (6 pm to 6 am) for the past 3 months
  • Able to complete a 3-month calendar of work schedule
Not Eligible

You will not qualify if you...

  • Known photosensitivity
  • Use of photosensitizing medications
  • Diabetes Mellitus
  • Use of hormonal agents such as birth control pills
  • History of abnormal scarring
  • History of skin infections
  • History of skin cancers
  • History of sleep disorders like sleep apnea or insomnia
  • Known allergy to lidocaine local anesthetic
  • Pregnancy or nursing
  • Other serious health issues

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

Diagnostic Evaluation

Duration - Single day

Participants undergo a skin punch biopsy where samples are exposed to ultraviolet B radiation or kept as non-irradiated controls in the laboratory.

1 visit (in-person)

Trial Site Locations

Total: 1 location

1

Wright State Physicians

Fairborn, Ohio, United States, 45324

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Research Team

M

Manager, Clinical Research Operations

R

Regulatory Specialist

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NON_RANDOMIZED

Model

PARALLEL

Primary Purpose

DIAGNOSTIC

Number of Arms

2

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