Study of How Smoking Amount Affects Saliva High Sensitive C-Reactive Protein Hs-CRP Levels Using Different Centrifuge Speeds and Times

What this Trial Is About

Researchers are studying how the amount of smoking affects levels of high sensitive C-reactive protein hs-CRP in saliva among healthy individuals with good periodontal health. The study compares saliva samples from non-smokers, light smokers less than 10 cigarettes per day, and heavy smokers more than 10 cigarettes per day to see how different centrifuge speeds and times impact hs-CRP measurement. Clinical periodontal parameters like probing depth, attachment level, bleeding on probing, gingival index, and plaque index are also recorded to explore correlations with smoking amount. Participants provide saliva samples by sitting upright and allowing unstimulated saliva to collect, which is then processed by centrifugation at different speeds and durations. The clear saliva portion is stored frozen until analysis. The study includes three groups based on smoking habits and involves detailed periodontal measurements for all. Samples are analyzed using enzyme-linked immunosorbent assay ELISA to measure hs-CRP levels. During the study, participants undergo full-mouth periodontal evaluations and saliva collection. Researchers measure total hs-CRP in saliva 24 hours after clinical measurements and determine the optimal centrifuge settings within one week after biochemical analysis. The study involves systemically and periodontally healthy adults aged 18 to 30 years, with no recent antibiotic, anti-inflammatory, or corticosteroid use, and no recent periodontal treatment. The overall goal is to better understand saliva hs-CRP levels in relation to smoking and centrifugation methods.

Effect of Different Centrifuge Values on Saliva High Sensitive C-Reactive Protein (Hs-CRP) Levels in Individuals With Different Smoking Amounts