Study of How Ephedrine, Phenylephrine, and Norepinephrine Affect Uterine Muscle Contraction in Pregnant Women With Type II and Gestational Diabetes During Cesarean Delivery

What this Trial Is About

Researchers are studying how medications used to treat low blood pressure during elective cesarean delivery CD affect the uterine muscles ability to contract, especially in women with Type II and gestational diabetes. These diabetic patients face a higher risk of postpartum hemorrhage due to possible changes in uterine contractility caused by vasopressors, which are drugs given to raise blood pressure during spinal anesthesia. The study aims to compare uterine contraction patterns and receptor distribution in diabetic and healthy pregnant women using tissue samples taken after CD. The study involves exposing small uterine muscle tissue samples to various vasopressorsephedrine, phenylephrine, and norepinephrinealone or combined with oxytocin. Samples are bathed in physiological salt solution with increasing drug concentrations to observe contractility changes. These experiments will explore how these medications affect the muscles contraction in diabetic versus healthy patients and analyze the receptors and pathways involved. Participants are pregnant women aged 19 to 45 undergoing elective cesarean delivery under spinal anesthesia, including those with Type II or gestational diabetes and healthy controls. After obtaining uterine tissue samples following delivery, researchers will measure contractility using motility index, amplitude, frequency, and area under the response curve over four hours. The study also monitors receptor expression and downstream pathways. Participation involves consenting to tissue sampling and data collection during standard CD procedures.

Effect of Ephedrine, Phenylepinephrine, and Norepinephrine on Myometrial Contractility in Pregnant People With Type II and Gestational Diabetes During Cesarean Section: An In-vitro Study