Study of ALPPS Surgery Effects and Mechanisms on Liver Regeneration in Patients With Liver Cancer

What this Trial Is About

Researchers are investigating the effects and mechanisms of the ALPPS procedure on liver regeneration in patients with hepatobiliary cancers, including hepatocellular carcinoma and cholangiocarcinoma. These cancers are aggressive and often diagnosed late. While liver resection offers a chance for cure, success depends on having enough healthy liver left after surgery. ALPPS is a newer surgical method that speeds up liver regrowth, allowing more patients to safely undergo liver removal compared to traditional approaches. The study aims to understand the cellular and genetic changes during liver regeneration caused by ALPPS and how these relate to patient outcomes over time. This observational study involves patients undergoing the ALPPS procedure at a hospital, with no changes made to their standard surgical care. ALPPS involves separating the liver and tying off part of the portal vein to stimulate rapid growth in the remaining liver. Researchers collect liver tissue and medical data during routine care and use advanced techniques like single-cell and spatial transcriptomics to analyze liver cell types and gene activity. The first stage is followed by assessment of liver volume growth before proceeding to the second surgery. Participants will have assessments of their liver volume around 7 and 14 days after the first ALPPS stage, with the main analysis using the last measurement before the second surgery. Researchers will monitor regeneration quality and relate it to short- and long-term clinical outcomes including recurrence. The study collects detailed clinical data and tissue samples without altering treatment, aiming to improve understanding of liver regrowth and inform future therapies. Participation duration depends on surgical timing and follow-up, with safety monitored through standard care.

Effect and Mechanism of ALPPS Operation on Liver Regeneration