Actively Recruiting

Phase Not Applicable
Age: 18Years - 65Years
All Genders
Healthy Volunteers
ID07388121

The Effect of Vitamin C Supplementation on Gut Microbiota Composition and Metabolic Activity in Healthy Adults

Led by University of Glasgow · Updated on 2026-02-04

23

Participants Needed

1

Research Sites

N/A

Total Duration

On this page

Sponsors

U

University of Glasgow

Lead Sponsor

P

Professor Konstantinos Gerasimidis, University of Glasgow

Collaborating Sponsor

AI-Summary

What this Trial Is About

This research aims to explore how vitamin C affects the bacteria living in the gut of healthy adults. Vitamins are essential nutrients that support various body functions, including the immune system and energy production. The study will investigate how gut bacteria interact with vitamin C and how this interaction influences their growth and activity, focusing on short-chain fatty acids and gut microbiota composition. Participants will follow their usual diet for one week, then take a moderate dose of vitamin C (200 mg daily) for two weeks, followed by a high dose (1000 mg daily) for another two weeks. After these supplementation periods, they will return to their habitual diet for one week. Vitamin C is provided as chewable tablets taken orally each day, and there is no washout period between phases. During the six-week study, participants will attend four study visits and a final follow-up stool sample collection. At each visit, measurements of body composition and collection of stool, blood, and urine samples will occur. Participants will complete food diaries, gastrointestinal symptom diaries, and compliance checklists. Researchers will measure gut microbiota activity, stool composition, vitamin C levels, inflammatory markers, and stool pH to understand the effects of vitamin C doses on gut health.

CONDITIONS

Brief Title

The Effect of Vitamin C Supplementation on Gut Microbiota Composition and Function in Healthy Adults

Who Can Participate

Age: 18Years - 65Years
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Healthy individuals aged 18 to 65 years with a BMI between 18.5 and 35 kg/m2
  • Self-reported good health with no chronic conditions requiring regular medical care
  • Willing to provide blood, urine, and stool samples at multiple time points
Not Eligible

You will not qualify if you...

  • Under 18 or over 65 years old
  • Current smoker
  • Chronic illness requiring regular medication or doctor visits
  • Current or recent medication affecting gut transit or digestion
  • History of major gastrointestinal surgery
  • Pregnant or breastfeeding
  • Regular use of prebiotics, probiotics, vitamins, or minerals unless willing to stop 2-4 weeks before study
  • Antibiotics taken in the past 3 months
  • Weight change greater than ±2 kg in the past month
  • Participation in other research likely to interfere with this study

AI-Screening

AI-Powered Screening

Complete this quick 3-step screening to check your eligibility

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 screening and enrollment visit (in-person)

Run-in Period

Duration - 1 week

Participants follow their usual habitual diet while baseline measurements and sample collections are taken.

1 visit at the start of the run-in period (in-person)

Moderate Vitamin C Supplementation

Duration - 2 weeks

Participants take a moderate dose of 200 mg vitamin C daily for two weeks while continuing their usual diet. Samples and measurements are collected at the start and end of this period.

2 visits (Week 1 and Week 3, in-person)

High-dose Vitamin C Supplementation

Duration - 2 weeks

Participants take a high dose of 1000 mg vitamin C daily for two weeks while continuing their usual diet. Samples and measurements are collected at the start and end of this period.

2 visits (Week 3 and Week 5, in-person)

Follow-up Period

Duration - 1 week

Participants resume their habitual diet without vitamin C supplementation. Final sample collection is done to observe any lasting effects.

1 visit for sample collection (Week 6, in-person)

Trial Site Locations

Total: 1 location

1

New Lister Building, Glasgow Royal Infirmary, 10-16 Alexandra Parade, G31 2ER

Glasgow, United Kingdom, G31 2ER

Actively Recruiting

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Research Team

R

Raihana Halim

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NON_RANDOMIZED

Model

SEQUENTIAL

Primary Purpose

BASIC_SCIENCE

Number of Arms

1

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