Exploring Tannin-Probiotic Interactions on Microbial Composition and Inflammatory Outcomes in Obesity

What this Trial Is About

This research aims to explore how gallotannin-rich mangoes and the probiotic Lactiplantibacillus pentosus affect inflammatory markers and gut microbiota in obese adults. The study focuses on whether these treatments, alone or combined, can reduce inflammation and change the gut bacteria linked to obesity. It also investigates if genetic differences in obese individuals influence how gallotannins are metabolized. The study includes both obese and lean participants to compare effects. Participants are divided into four groups: one receiving mango juice high in gallotannins, one receiving mango plus the probiotic, one receiving the probiotic alone, and a lean control group receiving no treatment. Stool samples from volunteers will be treated in the lab with these interventions to assess their impact. This is a randomized, double-blinded, placebo-controlled trial with a single sample collection visit during week 1. Participants will provide biological samples including saliva, blood, and stool to measure inflammatory and metabolic biomarkers, genetic markers, and gut microbiome composition. Advanced lab techniques like DNA sequencing and chemical analysis will be used to analyze changes. The main outcome is the level of gallotannin metabolites and short-chain fatty acids along with gut microbiome changes after 12 hours of in vitro treatment. The trial will help understand how these dietary interventions affect obesity-related inflammation and gut health.

Effects of Gallotannins on the Gut Microbiome and How it Affects Inflammatory Markers Among Obese Individuals.