Actively Recruiting

Phase 3
Age: 12Years - 35Years
All Genders
ID05477563

A Phase 3b Study to Evaluate Efficacy and Safety of a Single Dose of Autologous CRISPR Cas9 Modified CD34+ Human Hematopoietic Stem and Progenitor Cells (CTX001) in Subjects With Transfusion-Dependent Beta-Thalassemia or Severe Sickle Cell Disease

Led by Vertex Pharmaceuticals Incorporated · Updated on 2026-03-23

26

Participants Needed

6

Research Sites

N/A

Total Duration

On this page

AI-Summary

What this Trial Is About

Researchers are evaluating the safety and effectiveness of a single dose of CTX001, an autologous CRISPR-Cas9 modified CD34+ human hematopoietic stem and progenitor cell therapy, in participants aged 12 to 35 years with transfusion-dependent beta-thalassemia (TDT) or severe sickle cell disease (SCD). This Phase 3b open-label study aims to understand how this gene-edited therapy may impact these serious blood disorders that require frequent transfusions or cause severe complications. Participants will receive one infusion of CTX001 through a central venous catheter after undergoing myeloablative conditioning with busulfan. The study focuses on a single-dose administration of these genetically modified stem cells targeting the BCL11A gene enhancer to potentially improve hemoglobin production. No placebo or comparison group is involved, and the intervention is delivered intravenously. During the study, participants will be closely monitored for up to 12 months after infusion, including assessments of fetal and total hemoglobin levels over time. Safety evaluations will track adverse events, serious adverse events, and transplant-related mortality. Researchers will also measure engraftment success, genetic modification presence in blood and bone marrow, transfusion needs, and rates of vaso-occlusive crises and hospitalizations for SCD. Participants will undergo laboratory tests and clinical evaluations to gather comprehensive data on treatment impact and safety throughout the study period.

CONDITIONS

Brief Title

Evaluation of Efficacy and Safety of a Single Dose of CTX001 in Participants With Transfusion-Dependent β-Thalassemia and Severe Sickle Cell Disease

Who Can Participate

Age: 12Years - 35Years
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Participants aged between 12 and 35 years
  • Eligible for autologous stem cell transplant as determined by the investigator
  • Diagnosed with transfusion-dependent beta-thalassemia (including documented homozygous or compound heterozygous beta-thalassemia) or severe sickle cell disease
  • For beta-thalassemia participants: history of at least 100 mL/kg/year or 10 units/year of packed red blood cell transfusions in the prior 2 years
  • For sickle cell disease participants: history of at least two severe vaso-occlusive crisis events per year for the previous two years prior to enrollment
Not Eligible

You will not qualify if you...

  • A willing and healthy 10/10 human leukocyte antigen (HLA)-matched related donor is available
  • Prior hematopoietic stem cell transplant
  • Clinically significant and active bacterial, viral, fungal, or parasitic infection
  • For beta-thalassemia participants: associated alpha-thalassemia with more than 1 alpha deletion or alpha multiplications
  • For sickle cell disease participants: history of untreated moyamoya syndrome or presence of moyamoya syndrome at screening
  • Other protocol-defined exclusion criteria may apply

AI-Screening

AI-Powered Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

Treatment

Duration - Up to 12 months

Participants receive a single intravenous infusion of CTX001 following myeloablative conditioning with busulfan.

Trial Site Locations

Total: 6 locations

1

New York Presbyterian Hospital - Morgan Stanley Children's Hospital

New York, New York, United States, 10032

Actively Recruiting

2

Levine Children's Hospital - Hematology

Charlotte, North Carolina, United States, 28203

Actively Recruiting

3

TriStar Medical Group Children's Specialists - Pediatric Oncology

Nashville, Tennessee, United States, 37203

Actively Recruiting

4

University Hospital Dusseldorf - Department of Pediatric Oncology, Hematology and Clinical Immunology

Düsseldorf, Germany

Actively Recruiting

5

IRCSS Ospedale Pediatrico Bambino Gesu - Dipartimento di Onco-Ematologia e Terapia Cellulare e Genica

Rome, Italy

Actively Recruiting

6

King Faisal Specialist Hospital & Research Centre - Riyadh - Hematology

Al Mathar Ash Shamali, Saudi Arabia

Actively Recruiting

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Research Team

M

Medical Information

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NA

Model

SINGLE_GROUP

Primary Purpose

TREATMENT

Number of Arms

1

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