Study of Immune Changes Before and After Splenectomy in Adults with Immune Thrombocytopenia

What this Trial Is About

This research aims to evaluate the immune status before and after splenectomy in patients with immune thrombocytopenia ITP who have not had a lasting response to multiple treatments. The study involves 30 ITP patients who still meet the diagnosis criteria before surgery, and it also includes healthy donors and patients with splenectomy due to other causes as control groups. The purpose is to better understand immune changes related to splenectomy in this condition. The study involves laparoscopic splenectomy performed on the 30 ITP patients after raising their platelet levels to a safe state. Blood samples will be collected from these patients at six time points one day before surgery, then 1 week, 1 month, 3 months, 6 months, and 12 months after surgery. A small amount of spleen tissue will be taken during surgery. Healthy donors and patients with splenectomy for hereditary spherocytosis or trauma will also provide blood or spleen tissue samples for comparison. Participants will have blood drawn multiple times to analyze immune cell populations, activation and proliferation of B and T lymphocytes, platelet apoptosis by cytotoxic T cells, macrophage phagocytosis of platelets, and cytokine levels using laboratory tests such as ELISA. The study will monitor immune changes for one year after splenectomy. The total involvement includes pre-surgery assessment, surgery, and follow-up blood tests at specified intervals to measure immune responses and safety.

Evaluation of Immune Status Before and After Splenectomy in Immune Thrombocytopenia Patients