Characterization of Febrile Disease Landscape in Cambodia Using Metagenomic Pathogen Sequencing

What this Trial Is About

Researchers are investigating vector-borne diseases, which are infections caused by bites from mosquitoes, flies, fleas, ticks, or other blood-feeding insects. These diseases contribute to nearly one million deaths annually and are increasing, especially in Southeast Asia. This study aims to better understand these illnesses in Cambodia, where they may account for about 10% of fevers, by identifying pathogens using advanced metagenomic sequencing techniques. Participants include people aged 2 months to 65 years who have a fever of at least 38 degrees Celsius or have been diagnosed with important infectious pathogens. Close contacts of infected individuals may also join. Participants provide information about their health, travel, sex, age, and residence. Blood samples are collected, and nasal swabs are taken if respiratory symptoms are present. Optional follow-up blood samples and nasal swabs may be taken within 1-2 weeks and/or up to 3 months after enrollment. During the study, participants complete questionnaires and undergo medical history screening. Children are weighed to ensure suitability for blood sampling. The researchers will analyze pathogen sequences from samples collected between day 0 and 2 of enrollment and assess immune reactions to insect saliva. This study includes long-term tracking through optional follow-up samples to better understand disease spread and impact in the region.

Febrile Disease Landscape in Cambodia Via Metagenomic Pathogen Sequencing