Actively Recruiting

Phase 1
Age: 18Years - 24Years
All Genders
NCT06506461

Gene Editing For Sickle Cell Disease

Led by St. Jude Children's Research Hospital · Updated on 2026-05-05

25

Participants Needed

1

Research Sites

401 weeks

Total Duration

On this page

Sponsors

S

St. Jude Children's Research Hospital

Lead Sponsor

N

National Heart, Lung, and Blood Institute (NHLBI)

Collaborating Sponsor

AI-Summary

What this Trial Is About

This study is being done to test the safety of a new treatment called gene editing in Sickle Cell Disease (SCD) patients and to see if a single dose of this genetically modified cellular product will increase the amount of a certain hemoglobin called fetal hemoglobin (HbF) and help reduce the symptoms of SCD. Primary Objective * To assess the safety of autologous infusion of clustered regularly interspaced palindromic repeats (CRISPR)/ CRISPR associated protein (Cas9)-edited CD34+ hematopoietic stem and progenitor cells (HSPCs) in patients with severe SCD. Secondary Objective * To assess the efficacy autologous infusion of CRISPR/Cas9 genome-edited CD34+ HSPCs into patients with severe SCD.

CONDITIONS

Official Title

Gene Editing For Sickle Cell Disease

Who Can Participate

Age: 18Years - 24Years
All Genders

Eligibility Criteria

Eligible

You may qualify if you...

  • Age 18 to 24.9 years.
  • Diagnosis of Sickle Cell Disease with Hb SS, Hb SB0, or Hb SB+ genotype.
  • History of 2 or more SCD-related vaso-occlusive events per year in the 2 years before screening, OR regular red blood cell transfusions (8 or more in 12 months) except when for stroke prevention.
  • History of failure, intolerance, or refusal of hydroxyurea therapy.
  • Eligibility for autologous stem cell transplant by investigator judgment.
  • Females of childbearing potential must agree to use contraception from mobilization start through 6 months post-infusion.
  • Males must agree to use contraception from mobilization start through 6 months post-infusion.
  • Willingness to participate in additional long-term follow-up study after trial completion.
Not Eligible

You will not qualify if you...

  • Availability of an HLA-matched sibling donor for hematopoietic cell transplantation.
  • Karnofsky or Lansky performance score below 80.
  • Pregnancy confirmed by positive test within 14 days before enrollment.
  • Breastfeeding.
  • Uncontrolled or clinically significant infections within 1 month before enrollment.
  • Confirmed Hepatitis B or C infection.
  • Confirmed HIV or HTLV infection.
  • History of stroke.
  • Serum conjugated bilirubin above 2 times normal or ALT above 3 times normal, unless values improve.
  • Left ventricular shortening fraction under 25% or ejection fraction under 45%.
  • Estimated creatinine clearance below 60 mL/min/1.73m².
  • Diffusion capacity of carbon monoxide under 50% or baseline oxygen saturation under 85% if unable to perform lung tests.
  • Prior hematopoietic cell transplant or gene therapy.
  • Known liver cirrhosis, bridging fibrosis, or active hepatitis.
  • Active malignancy, myelodysplasia, abnormal cytogenetics, or immunodeficiency.
  • History of significant bleeding disorder.
  • Cerebrovascular procedure within 6 months.
  • Untreated or risky moyamoya disease.
  • Presence of pathogenic clonal mutations linked to blood cancers.
  • Intolerance or allergy to plerixafor, motixafortide, or busulfan.
  • Participation in another investigational drug study within 30 days or less than 5 half-lives of the drug.
  • History of alloimmunization to red blood cell antigens with anticipated insufficient blood units for study duration.

AI-Screening

AI-Powered Screening

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Trial Site Locations

Total: 1 location

1

St. Jude Children's Research Hospital

Memphis, Tennessee, United States, 38105

Actively Recruiting

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Research Team

A

Akshay Sharma, MBBS, MSc

CONTACT

How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NA

Model

SINGLE_GROUP

Primary Purpose

TREATMENT

Number of Arms

1

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