Actively Recruiting

Phase Not Applicable
Age: 18Years +
All Genders
Healthy Volunteers
ID06307171

Human Leishmaniasis: Antigen Recognition Pattern and Study of New Potential Biomarkers

Led by IRCCS Sacro Cuore Don Calabria di Negrar · Updated on 2024-03-12

170

Participants Needed

1

Research Sites

N/A

Total Duration

On this page

AI-Summary

What this Trial Is About

Researchers are studying Leishmaniasis, a parasitic infection, to identify specific biomarkers that could improve blood tests for detecting the disease. The study aims to find biomarkers that do not cross-react with other infections such as Chagas, tuberculosis, leprosy, or malaria. It also examines the Antigen Recognition Patterns (ARPs) of Leishmania infection and whether these patterns apply to different strains from Italy, Sudan, and other regions. The study involves analyzing stored and newly collected blood and serum samples from patients with confirmed Leishmaniasis, uninfected individuals, and those with other infections. Researchers use a specialized diagnostic test called Western blot to examine different protein fractions from Leishmania strains. They identify specific immunogenic proteins through laboratory techniques including mass spectrometry. Participants contribute blood samples that are tested and analyzed for biomarkers and ARPs. The study reviews these patterns at baseline to understand cross-reactions with other diseases. Samples come from multiple groups, including Leishmaniasis patients, healthy controls, and individuals with other infections. The research is observational with no assigned treatments, and participation involves providing samples and medical data. The study is expected to continue until November 2026.

CONDITIONS

Brief Title

Human Leishmaniasis: Antigen Recognition Pattern and Study of New Potential Biomarkers

Who Can Participate

Age: 18Years +
All Genders
Healthy Volunteers

Eligibility Criteria

Eligible

You may qualify if you...

  • Adults aged 18 years or older
  • Patients diagnosed with Leishmaniasis from Italy or Sudan confirmed by clinical or laboratory tests
  • Signed informed consent or use of stored samples with consent
  • Classified as leishmaniasis patients by clinicians or positive to at least two different serological or molecular tests
  • Cryo-preserved serum samples from uninfected individuals with negative results to two serological tests
  • Serum samples from subjects diagnosed with Chagas disease, tuberculosis, malaria, or leprosy stored in the Tropica Biobank
  • Signed informed consent for Tropica Biobank samples
Not Eligible

You will not qualify if you...

  • Lack of necessary medical data or biological samples
  • Individuals younger than 18 years
  • Positive result to any serological test for Leishmaniasis in community controls or validation controls

AI-Screening

AI-Powered Screening

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Your Study Journey

Screening

Duration - 2 to 4 weeks

Participants are screened for eligibility to participate in the trial.

1 visit (in-person)

Diagnostic Evaluation

Duration - Baseline

Participants provide serum samples which are tested using Western blot and other serological tests to identify antigen recognition patterns and potential biomarkers for Leishmaniasis.

1 visit (in-person) for sample collection

Trial Site Locations

Total: 1 location

1

IRCCS Sacro Cuore Don Calabria hospital

Negrar, Verona, Italy, 37024

Actively Recruiting

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How is the study designed?

Study Type

INTERVENTIONAL

Masking

NONE

Allocation

NON_RANDOMIZED

Model

SINGLE_GROUP

Primary Purpose

DIAGNOSTIC

Number of Arms

3

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