Study of White Blood Cell Responses to Inflammatory Signals in Healthy Adults Ages 18 to 65 with Blood Donation

What this Trial Is About

Researchers are investigating how certain white blood cells, such as neutrophils and monocytes, respond to signals in the body and what substances they produce. This study focuses on understanding how these cells might contribute to inflammation and measures substances in blood plasma that could stimulate white blood cells. The research particularly looks at signaling pathways activated by lipopolysaccharide LPS and other immune stimuli in human leukocytes in laboratory tests. Healthy adult volunteers aged 18 to 65 donate up to 320 milliliters of blood no more than once every 8 weeks, with a maximum of six donations per year. The collected blood is separated into its components, and immune cells are exposed to inflammatory substances in the lab to observe their responses, including chemical signaling and functional activities. The study also examines the role of cholesterol-rich areas in cell membranes, the tumor suppressor gene p53s influence on inflammation, and zinc finger proteins effects on gene expression in inflammatory cells. Participants undergo blood donation in a monitored setting, and their blood samples are tested for HIV and hepatitis BC every 6 months to ensure safety. Researchers analyze various immune cell responses to inflammatory stimuli, including gene expression and signaling pathways. The study aims to better understand inflammation mechanisms by measuring these responses in vitro. Participation involves multiple blood donations over time, with careful blood screening and monitoring of donor health throughout the study.

Immune Cell Response to Stimuli