Immunometabolic Mechanisms of Blood Flow Restriction Training After Anterior Cruciate Ligament Reconstruction

What this Trial Is About

Researchers are investigating how blood flow restriction BFR training affects immune cell metabolism and gene expression in people recovering from anterior cruciate ligament ACL reconstruction. This phase 4 study explores the impact of BFR on immunometabolism during rehabilitation to better understand its biological effects. The study uses a single-blind crossover design where participants experience both BFR and control sessions in random order. Participants will attend two training sessions at least one week apart. In one session, an AirBand device will be inflated to restrict blood flow by 60 in the femoral artery while an ultrasound probe monitors the artery. In the other session, participants will wear an uninflated AirBand as a control. A certified Personal Therapist will supervise all sessions to ensure safety and adherence. Throughout each session and at baseline, immediately after, and at 30 and 60 minutes post-exercise, researchers will measure changes in leukocyte metabolic gene expression, substrate preference, and concentrations of amino acids, glucose, lactate, fatty acids, insulin, glucagon, and catecholamines. Secondary measures include creatine kinase levels, patient-reported soreness, and baseline immunometabolic profiles. The total study duration involves two visits with monitoring of biological and self-reported outcomes.

Immunometabolic Mechanisms of Blood Flow Restriction (BFR) Training After Anterior Cruciate Ligament Reconstruction