Study of Omega-3 Monoglycerides Effects on Blood Cells to Help Resolve Inflammation

What this Trial Is About

Researchers are investigating the potential beneficial effects of omega-3 monoglyceride fatty acids on reducing inflammation by studying their impact on peripheral blood mononuclear cells PBMCs. This research focuses on the biological activity of omega-3 fatty acids, which involves selective oxidation by enzymes called lipoxygenases to form substances that help resolve inflammation. The study aims to develop new products by pre-metabolizing omega-3 monoglycerides to enhance this inflammation-resolving activity. The study collects blood samples from participants to isolate PBMCs, which are then cultured with an inflammatory agent called LPS to stimulate cell proliferation. The cells are divided into two groups one treated with the specially processed omega-3 monoglycerides MAG-SPM and the other untreated with MAG-SPM but exposed to LPS. Researchers measure and compare the reduction in cell proliferation between these two groups to assess the activity of the treatment. Participants donate blood samples used for cell isolation. The isolated cells are maintained in laboratory cultures with LPS, and their proliferation is observed with and without MAG-SPM treatment. Researchers count the number of PBMCs over a 12-month period to evaluate the inflammation-resolving effects. This study involves regular laboratory assessments and does not involve direct treatment of participants. Participation duration depends on the blood donation schedule and laboratory testing timeline.

Increased Inflammation-resolving Activity by Omega-3 Monoglycerides in Peripheral Blood Mononuclear Cells (PBMC).