IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
Daniel T Fisher, Qing Chen, Joseph J Skitzki...
https://pubmed.ncbi.nlm.nih.gov/21926464Actively Recruiting
Phase Not Applicable
Age: 18Years +
All Genders
ID03823144
Intravital Microscopy (IVM) in Human Solid Tumors
Led by Mayo Clinic ยท Updated on 2026-06-02
85
Participants Needed
1
Research Sites
N/A
Total Duration
On this page
AI-Summary
What this Trial Is About
Researchers are exploring the blood vessels associated with solid tumors in adult patients using a method called human intravital microscopy (HIVM). This study aims to see if HIVM can be done safely during surgery to remove tumors and to learn how tumor blood vessels differ from normal tissue. The study will also examine if these vessel characteristics relate to tumor grade and patient survival. Participants receive an intravenous injection of fluorescein dye during surgery, and HIVM is performed for 1-2 minutes to view the tumor vessels in real time. The study focuses on tumors from various cancers including gastric, pancreatic, liver, colorectal, sarcoma, brain, and breast cancers. After surgery, patients are followed up 2-3 weeks later. During the study, researchers will measure tumor vessel number, density, dye uptake, and blood flow using HIVM. They will compare tumor vessels to normal tissue vessels and correlate these findings with tumor pathology and survival over up to five years. The total involvement includes surgery, HIVM imaging, and post-operative follow-up assessments.
CONDITIONS
Brief Title
Intravital Microscopy in Human Solid Tumors
Who Can Participate
Age: 18Years +
All Genders
Eligibility Criteria
You may qualify if you...
- Age 18 years or older
- ECOG Performance Status of 2 or less
- Measurable tumor requiring surgical removal in the operating room
- Tumor types include gastric, pancreatic, hepatobiliary, colorectal, sarcoma, brain, or breast cancer, primary or metastatic
- Ability to understand and sign informed consent
- Negative skin prick test for fluorescein sensitivity before surgery
You will not qualify if you...
- Uncontrolled illness such as active infection, heart failure, unstable angina, arrhythmia, or psychiatric/social issues
- Kidney dysfunction with GFR less than 45
- Liver dysfunction with Child-Pugh score above 5 or liver tests 1.5 times above normal
- Known allergy or prior reaction to fluorescein or positive skin test
- Pregnant or nursing women
- Unable or unwilling to follow study procedures
- Conditions making patient unsuitable or needing further cardiac evaluation
- Conditions excluding surgical tumor removal as standard care
AI-Screening
AI-Powered Screening
Complete this quick 3-step screening to check your eligibility
1
2
3
Your Study Journey
Screening
Duration - 2 to 4 weeks
Participants are screened for eligibility to participate in the trial.
1 visit (in-person) for eligibility screening including a skin prick test for fluorescein sensitivity
Diagnostic Evaluation
Duration - Surgical day
Participants receive fluorescein intravenously and undergo Human Intravital Microscopy (HIVM) during surgical tumor resection, allowing real-time visualization of tumor-associated blood vessels for approximately 12-15 minutes.
1 visit coinciding with the surgical tumor removal
Post-operative Follow-up
Duration - 2 to 3 weeks after surgery
Participants are followed up to compare microvasculature observations with normal tissue and correlate findings with tumor pathology and survival.
1 follow-up visit approximately 2 to 3 weeks after surgery
Trial Site Locations
Total: 1 location
1
Mayo Clinic Florida
Jacksonville, Florida, United States, 32224
Actively Recruiting
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Research Team
E
Emmanuel M Gabriel, M.D., Ph.D.
M
Michael B Wallace, M.D.
How is the study designed?
Study Type
INTERVENTIONAL
Masking
NONE
Allocation
NA
Model
SINGLE_GROUP
Primary Purpose
DIAGNOSTIC
Number of Arms
1
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Published Research Related To This Trial
Intraoperative intravital microscopy permits the study of human tumour vessels.
Daniel T Fisher, Jason B Muhitch, Minhyung Kim...
https://pubmed.ncbi.nlm.nih.gov/26883450Heterogeneity of the tumor vasculature.
Janice A Nagy, Sung-Hee Chang, Shou-Ching Shih...
https://pubmed.ncbi.nlm.nih.gov/20490982Evading tumor evasion: current concepts and perspectives of anti-angiogenic cancer therapy.
Amir Abdollahi, Judah Folkman
https://pubmed.ncbi.nlm.nih.gov/20061178Tumor microvasculature and microenvironment: novel insights through intravital imaging in pre-clinical models.
Dai Fukumura, Dan G Duda, Lance L Munn...
https://pubmed.ncbi.nlm.nih.gov/20374484Primary immune surveillance: some like it hot.
Joseph J Skitzki, Qing Chen, W C Wang...
https://pubmed.ncbi.nlm.nih.gov/17704903Dissecting tumour pathophysiology using intravital microscopy.
Rakesh K Jain, Lance L Munn, Dai Fukumura
https://pubmed.ncbi.nlm.nih.gov/12001988Multiphoton intravital microscopy to study lymphocyte motility in lymph nodes.
Thomas T Murooka, Thorsten R Mempel
https://pubmed.ncbi.nlm.nih.gov/21909917Imaging tumor cell movement in vivo.
David Entenberg, Dmitriy Kedrin, Jeffrey Wyckoff...
https://pubmed.ncbi.nlm.nih.gov/23456602Imaging of human lymph nodes using optical coherence tomography: potential for staging cancer.
Robert A McLaughlin, Loretta Scolaro, Peter Robbins...
https://pubmed.ncbi.nlm.nih.gov/20233873