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This research aims to evaluate how eco-driving assistance EDA systems affect the health and well-being of professional bus drivers. The study investigates whether EDA acts as a stressor and whether drivers experience increased physiological and psychological stress when driving buses equipped with EDA. Researchers will also explore the link between stress measurements and biomarkers found in biological samples. Participants will be randomly assigned to drive buses equipped with eco-driving assistance or buses without it, using a within-subject design so each driver experiences both conditions. During their working days, drivers will wear a wearable electrocardiogram ECG device to record heart rate variability HRV. They will also complete a questionnaire at the end of each shift, including additional questions if they drove an EDA-equipped bus. Participants must complete at least 5 hours of driving with EDA and 5 hours without to finish the study. Throughout the study, participants heart rate variability and perceived stress levels will be closely monitored. When driving EDA-equipped buses, drivers will review reports generated by the EDA system and answer related questions. Biological samples and physiological measurements will be collected upon consent. The study lasts until the required driving hours are met, with a maximum duration of 14 days.